Study of sorption and toxicological properties of porous silicon nanoparticles with precipitated cinnarizine
Abstract
Low solubility, leading to insignificant dissolution in the gastrointestinal tract, is the main problem for drugs intended for oral use. One of such drugs is cinnarizine. Cinnarizine drugs on the pharmaceutical market are represented by immediate-release drugs, characterized by low absorption and low bioavailability. In order to increase solubility, the issue of using silicon nanoparticles is currently relevant.
The purpose of this study is to study the features of sorption-desorption of cinnarizine from the surface of porous silicon, to study the acute and chronic toxicity of porous silicon nanoparticles without cinnarizine and with precipitated cinnarizine, in the functional load test on ciliates Paramecium caudatum.
Samples of macro- and mesoporous silicon were obtained by two-sided anodic electrochemical etching of single-crystal silicon. After mechanical and ultrasonic grinding of the porous layer, the average size of silicon nanoparticles was ~10-20 nm.
The processes of sorption-desorption of cinnarizine from the surface of macro- and mesoporous silicon were studied by dialysis through a semipermeable membrane. The optimal time of adsorption of cinnarizine on the surface of macro- and mesoporous silicon was 30 minutes. When studying the desorption of cinnarizine from the surface of por-Si, it was found that after 45 minutes of the experiment, the concentration of cinnarizine in a medium of 0.1 M hydrochloric acid was 0.73 and 0.51 mg/ml, respectively. When studying the acute and chronic toxicity of porous silicon nanoparticles with precipitated cinnarizine on a test object from the type of protozoan ciliates Paramecium caudatum, it was found that mesoporous silicon nanoparticles increase the viability of paramecia in a functional load test without exhibiting toxic effects towards them, while mesoporous silicon nanoparticles with precipitated cinnarizine can reduce the viability of ciliates.
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