The development of HPLC determination of aciclovir’s fragment in new pharmaceutical substance acyclogermanium
Abstract
The objects of the study was new active pharmaceutical ingredient acyclogermanium
(Ge2[C6H5O7]4[C8H11N5O3][C6H14N4O2]), organometallic germanium compound with aciclovir’s fragment. A
simple, rapid, and selective HPLC method was developed for the estimation of acyclovir’s fragment in
pharmaceutical substance and dosage form (water-soluble gel). The chromatographic behavior of the
aciclovir was studied by HPLC on a reverse phase C18 column with spectrophotometric detection with
recourse to eluent phosphate buffer (pH 2.5) – acetonitrile, 96:4, at a flow rate of 1.0 mL/min with UV
detection at 254±4 nm. The retention time of acyclovir was 3.5 minutes. The purity of the compound was
also studied. The main impurity is detected and identified as guanine by UV-spectrophotometry and massspectrometry
.
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References
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