Low-pressure chromatograph for protein separation

Abstract

The paper is devoted to the study of the capabilities of a new domestic laboratory low-pressure chromatograph designed for the separation and preparative isolation of proteins by various chromatographic methods. The equipment operates in isocratic and gradient modes using ultraviolet and conductometric detectors. A built-in fraction collector with a changing valve makes it possible both to collect a target fraction and to run a cyclic program for the production of the substance with intermediate regeneration of the chromatographic column. The sample can be either injected or pumped in using a separate line. The domestic software package Multichrom Axioma is used to control the equipment and to record data.

The conducted study showed that the device allows implementing all the main methods of protein separation: ion exchange, hydrophobic, affinity, and size-exclusion chromatography. The paper demonstrates the scope of use of the equipment depending on the type of chromatographic separation and the size of the used columns.