RP HPLC determination of some uncommon anthocyanins
Keywords:
Reversed-phase HPLC, uncommon anthocyanins, 7-OH methylation; 5-OH methylation; regularities of retention alteration, electronic spectra.
Abstract
Anthocyanins of Catharanthus roseus petals were found to be composed of pairs (3-galactosides
and 3-rhamnosylgalactosides) mainly of 7-methylated anthocyanidins of “dephinidin series”
(7-methyldelphinidin, 7-methylpetunidin and hirsutidin); though the same derivatives of “cyanidin series” are
present in low concentrations. Flowers of Caesalpinia pulcherrima contain two 3-glucosides: of cyanidin and
5-methylcyanidin. The migration of CH3-radical from 3’-OH group to 7-OH position results in increase of
retention, while for the migration to 5-OH group a decrease of retention was observed. Different position of
ring A OH-groups methylation can be predicted also by controversial shift of λmax of the solutes.
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References
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K.M., Winefield C. Springer. 2009. 330pp.
2. Natural colorants for food and nutraceutical uses / by F. Delgado-Vargas and Octavio
P.-L. CRC Press LLC. 2003. 327 pp.
3. Berardini N., Schieber A., Klaiber I., Beifuss U. et al. B 7-O-Methylcyanidin 3-O-β-
D-Galactopyranoside, a Novel Anthocyaninfrom Mango (Mangifera indica L. cv. ‘Tommy
Atkins’) Peels // Z. Naturforsch. 2005. Vol. 60b. P. 801-804.
4. Deineka V.I., Grigor’ev A.M. Determination of anthocyanins by high-performance
liquid chromatography: regularities of retention // J. Anal. Chem. 2004. Vol. 59.
P. 305-309.
5. Deineka V.I. Comparative Analysis of HPLC Retention: A Thermodynamic Approach
to the Interpretation of Correlation Equations // Russian J. Phys. Chem. 2006. Vol. 80.
P. 1172–1174.
6. Piovan A., FilippiniR. Anthocyanins in Catharanthus roseus in vivo and in vitro: a
review // Phytochem. Rev. 2007. Vol.6. P. 235-242.
7. Toki K., Saito N., Irie Y., Tatsuzawa F., Shigihara A., Honda T. 7-O-Methylated
anthocyanidin glycosides from Catharanthus roseus // Phytochemistr. 2008. Vol. 69.
P. 1215-1219.
8. Deyneka V.I. , Grigor'yev A.M. , Deyneka L.A. , Shaposhnik Ye.I. , Staroverov V.M.
Issledovaniye antotsianov cherniki v plodakh i preparatakh na yeye osnove / / Zav . lab .
2006. № 3 . P. 16-20 . [in Russian]
Published
2019-11-19
How to Cite
Deineka, V. I., Vu Thi, N. A., & Deineka, L. A. (2019). RP HPLC determination of some uncommon anthocyanins. Sorbtsionnye I Khromatograficheskie Protsessy, 14(3). Retrieved from https://journals.vsu.ru/sorpchrom/article/view/1488
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