Extraction technologies for clinical HPLC analysis of serum corticosteroids
Keywords:
cortisol, cortisone, secondary steroids, solid-phase extraction (SPE), hyper crosslinked polystyrene, HPLC.
Abstract
The method solid-phase extraction (SPE) cortisol, cortisone and secondary steroids from human
serum for the subsequent analysis with conventional reversed-phase HPLC and UV detection is offered.
SPE on cartridges packed 30 mg hyper cross-linked polystyrene. Simplicity, reproducibility both
sufficient selectivity and sensitivity of a method, allow to use it in a clinical practice for diagnostics
congenital adrenal hyperplasia.
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References
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chromatography with ultraviolet detection. // J. Chromatogr.-B. 1992. V. 584. P. 147-155.
10. Карцова Л.А., Бессонова Е.А., Объедкова Е.В., Даванков В.А. Использование
сверхсшитого полистирола как сорбента для твердофазной экстракции при анализе лекарств
в биологических объектах методом высокоэффективной тонкослойной хроматографии
(ВЭТСХ). // Сорбционные и хроматографические процессы. 2010. Т. 10. Вып. 1. c. 5-14.
progestins in serum. // J. Chromatogr. - B. 1987. V. 421. P. 123-129.
2.Kabra PM. Clinical analysis of individual steroids by column liquid chromatography (review).
//J. Chromatogr.-B. 1988. V. 429. P. 155-176.
3.Volin P. High-performance liquid chromatographic analysis of corticosteroids (review). // J. of
Chromatogr. B. 1995. V. 671. P. 319-340.
4.Steroid Analysis. Edited by H.L.J. Makin and D.B. Gower. Springer. 2010. 1224 р.
5.Shackleton C. Clinical steroid mass spectrometry: a 45-year history culminating in HPLCMS/
MS becoming an essential tool for patient diagnosis. // J. Steroid Biochem Mol. Biol., 2010. V.
121(3-5). P. 481-490.
6.Fanelli F., Belluomo I., Di Lallo V.D., Cuomo G., De Iasio R., Baccini M., Casadio E., Casetta
B., Vicennati V., Gambineri A., Grossi G., Pasquali R., Pagotto U. Serum steroid profiling by
isotopic dilution-liquid chromatography-mass spectrometry: comparison with current
immunoassays and reference intervals in healthy adults. // Steroids. 2011. V. 76(3). P. 244-253.
7.Sjovall J., Axelson M. Never approaches to the isolation, identification and quantitation of
steroid in biological material (review). // Vitamins and Hormones. 1982. V. 39. P. 31-41.
8.Shackleton CHL. Profiling steroid hormones and urinary steroids (review). //J. Chromatogr. -
B. 1986. V. 379. P. 91-156.
9.Volin P. Simultaneous determination of serum cortisol and cortisone by reversed-phase liquid
chromatography with ultraviolet detection. // J. Chromatogr.-B. 1992. V. 584. P. 147-155.
10. Карцова Л.А., Бессонова Е.А., Объедкова Е.В., Даванков В.А. Использование
сверхсшитого полистирола как сорбента для твердофазной экстракции при анализе лекарств
в биологических объектах методом высокоэффективной тонкослойной хроматографии
(ВЭТСХ). // Сорбционные и хроматографические процессы. 2010. Т. 10. Вып. 1. c. 5-14.
Published
2019-11-22
How to Cite
Dutov, A. A., Nikitin, D. A., Sverkunova, A. V., Martynova, A. V., Konovalova, O. N., Fedorova, E. N., Lukyanova, J. L., & Yermolina, A. V. (2019). Extraction technologies for clinical HPLC analysis of serum corticosteroids. Sorbtsionnye I Khromatograficheskie Protsessy, 13(6). Retrieved from https://journals.vsu.ru/sorpchrom/article/view/1714
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