Appliance of sorbent-based extraction method to optimize rat liver tissue DNA isolation protocol
Keywords:
DNA, phenol-chlorophorm extraction, silica sorbent, electrophoresis.
Abstract
Purity of nucleic acids, the way they were obtained and nucleic acid yield are crucial for many
molecular biology techniques, studying the physicochemical properties of nucleic acids and chemical
modification. Though pnenol-chlorophorm-based extraction of DNA remains traditional, other
approaches are also widely-used, such as magnetic particles, chromatography or sorbents usage. The
appliance of two different techniques (pnenol-chlorophorm extraction and sorbent-based isolation) is
observed in the present article
Downloads
Download data is not yet available.
References
1. Milligan BG. Total DNA isolation // Oxford University Press. 1998. pp. 29–64.
2. Hong Chen, Murugesan Rangasamy, Sek Yee Tan, Haichuan Wang, В. D. Siegfried.
Evaluation of Five Methods for Total DNA Extraction from Western Corn Rootworm
Beetles // PLoS One. 2010. 5(8).
3. Lutz K, Wenqin W., Zdepski А., Michael Т. Isolation and analysis of high quality
nuclear DNA with reduced organellar DNA for plant genome sequencing and resequencing
// BMC Biotechnol. 2011; 11: 54.
4. Bravo D., Clari M., Costa E., Muñoz-Cobo B., Solano C., Remigia M.J., Navarro D.
Comparative Evaluation of Three Automated Systems for DNA Extraction in Conjunction
with Three Commercially Available Real-Time PCR Assays for Quantitation of Plasma
Cytomegalovirus DNAemia in Allogeneic Stem Cell Transplant Recipients // J Clin
Microbiol. 2011. 49(8): 2899–2904.
5. Chomczynski P., Sacchi N.. The single-step method of RNA isolation by acid
guanidinium thiocyanate–phenol–chloroform extraction: twenty-something years on //
Nature protocols. 2006. vol.1. no.2.
6. Rogers S.O., Bendich A. J. Extraction of DNA from milligram amounts of fresh,
herbarium and mummified plant tissues // Plant Molecular Biology. 1985. Vol. 5. 69-76.
7. Kay E., Simmons N., Alexander L. An Improved Preparation of Sodium
Desoxyribonucleate // J. Am. Chem. Soc. 1952. Vol. 74 (7). 1724–1726.
2. Hong Chen, Murugesan Rangasamy, Sek Yee Tan, Haichuan Wang, В. D. Siegfried.
Evaluation of Five Methods for Total DNA Extraction from Western Corn Rootworm
Beetles // PLoS One. 2010. 5(8).
3. Lutz K, Wenqin W., Zdepski А., Michael Т. Isolation and analysis of high quality
nuclear DNA with reduced organellar DNA for plant genome sequencing and resequencing
// BMC Biotechnol. 2011; 11: 54.
4. Bravo D., Clari M., Costa E., Muñoz-Cobo B., Solano C., Remigia M.J., Navarro D.
Comparative Evaluation of Three Automated Systems for DNA Extraction in Conjunction
with Three Commercially Available Real-Time PCR Assays for Quantitation of Plasma
Cytomegalovirus DNAemia in Allogeneic Stem Cell Transplant Recipients // J Clin
Microbiol. 2011. 49(8): 2899–2904.
5. Chomczynski P., Sacchi N.. The single-step method of RNA isolation by acid
guanidinium thiocyanate–phenol–chloroform extraction: twenty-something years on //
Nature protocols. 2006. vol.1. no.2.
6. Rogers S.O., Bendich A. J. Extraction of DNA from milligram amounts of fresh,
herbarium and mummified plant tissues // Plant Molecular Biology. 1985. Vol. 5. 69-76.
7. Kay E., Simmons N., Alexander L. An Improved Preparation of Sodium
Desoxyribonucleate // J. Am. Chem. Soc. 1952. Vol. 74 (7). 1724–1726.
Published
2019-11-25
How to Cite
Bashmakov, V. Y., Solodskikh, S. A., Panyovina, A. V., Shmatkova, M. L., & Popov, V. N. (2019). Appliance of sorbent-based extraction method to optimize rat liver tissue DNA isolation protocol. Sorbtsionnye I Khromatograficheskie Protsessy, 12(5). Retrieved from https://journals.vsu.ru/sorpchrom/article/view/1862
Section
Статьи
