Studiyng of guanidine isothiocyanate for express RNA extraction coupled with polymerase chain reaction analysis of cell bioenergetics

  • Maria N. Agafonova assistant at Institute Of Chemistry named after A.M. Butlerov, Kazan Federal University, Kazan, dekanat7@mail.ru
  • Ekaterina V. Kostromicheva assistant at Dep. Of Biotechnology, Faculty Of Biotechnology and Veterinary, Orel State Agricultural University, Orel
  • Ilya V. Kublanov jr. researcher at RAS Institute Of Microbiology named after S.N. Vinogradsky, Moscow
  • Olga P. Lebedeva docent at Department of Obstetrics and Gynecology, Faculty Of Medicine, National Research University Belgorod State University, Belgorod
  • Konstantin G. Lyamzaev researcher at The A.N. Belozersky Institute Of Physico-Chemical Biology, Moscow
  • Olga A. Podosokorskaya jr. researcher at RAS Institute Of Microbiology named after S.N. Vinogradsky, Puschino,
  • Anna A. Popova PhD student at Genetics, Cytology and Bioengineering Dep., Faculty of Biology and Soil Sciences, Voronezh State University, Voronezh
  • Natalya A. Rudykh docent at Department of Obstetrics and Gynecology, Faculty Of Medicine, National Research University Belgorod State University, Belgorod
  • Maria A. Simonova PhD student at Institute Of Theoretical And Experimental Biophysics of RAS, Puschino
  • Svetlana S. Sirotina PhD student at Biochemical faculty of National Research University Belgorod State University, Belgorod
  • Maria N. Tutukina researcher at Institute Of Cell Biophysics of RAS, Puschino
  • Vasily N. Popov professor, Dr. Biol., Head of Genetics, Cytology and Bioengineering Dep., Faculty of Biology and Soil Sciences, Voronezh State University, Voronezh
  • Victor Yu. Bashmakov PhD student at Genetics, Cytology and Bioengineering Dep., Faculty of Biology and Soil Sciences, Voronezh State University, Voronezh
Keywords: guanidine isothiocyanate, RNA, real-time PCR.

Abstract

Studiyng the conditions of RNA extraction revealed the guanidine isothiocyanate protocol to be
the most effective for RNA extraction from blood cells. The current study highlights the importance of
microsurrounding maintenance while gene expression analysis is in progress. It was shown that primer
melting temperature and MgCl2 concentration adjustment is essential for PCR detection of certain
sequences optimization

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Published
2019-11-25
How to Cite
Agafonova, M. N., Kostromicheva, E. V., Kublanov, I. V., Lebedeva, O. P., Lyamzaev, K. G., Podosokorskaya, O. A., Popova, A. A., Rudykh, N. A., Simonova, M. A., Sirotina, S. S., Tutukina, M. N., Popov, V. N., & Bashmakov, V. Y. (2019). Studiyng of guanidine isothiocyanate for express RNA extraction coupled with polymerase chain reaction analysis of cell bioenergetics. Sorbtsionnye I Khromatograficheskie Protsessy, 12(6). Retrieved from https://journals.vsu.ru/sorpchrom/article/view/1890
Issue
Vol 12 No 6 (2012): Sorbtsionnye i Khromatograficheskie Protsessy
Section
Статьи