Chromatographic behavior of amino acid derivatives obtained with o-phthalic aldehyde and various nucleophilic agents
Keywords:
high performance liquid chromatography, amino acids, o-phthalic aldehyde, nucleophilic agents.
Abstract
The chromatographic separation of major amino acids on the «Zorbax Eclipse AAA»
column after derivatization with o- phthaldialdehyde and various thiol compounds was studied. The
advantage of using N-isobutiryl-L-cysteine was established. Using this reagent allows one to avoid the
decrease of separation selectivity while following the technique suggested by column producer. The
correlations between the capacity factor and hydrophobicity coefficient providing the ability to predict the
order of sorbates elution were obtained
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References
1. H. Bruckner, M. Langer, M. Lupke, T. Westhauser, H. Godel. Liquid chromatographic
determination of amino acidenantiomers by derivatization with o-phthaldialdehyde and
chiral thiols. Applications with reference to food science // Journal of Chromatography A.
1995. V 697. P 229-245.
2. Чернобровкин М.Г., Шаповалова Е.Н., Шпигун О.А., Ананьева И.А.
Определение энантиомеров аминокислот в фармацевтических препаратах методом
обращенно-фазовой ВЭЖХ // Журн. аналит. химии. 2004. Т.59. №1. С. 64-73.
3. S. L. Grant, Y. Shulmanb, P. Tibbo, D. R. Hampson, G. B. Bakera, Determination of
d-serine and related neuroactive amino acids in human plasma by high-performance liquid
chromatography with fluorimetric detection // J. Chromatogr B. 2006. V. 844. P. 278-282.
4. I. Pérez-Neria, E. Castrob, S. Montesa, M.-C. Bolla, J. Barges-Coll, José Luis Soto-
Hernándezd, Camilo Ríos. Arginine, citrulline and nitrate concentrations in the
cerebrospinal fluid from patients with acute hydrocephalus. // J. Chromatogr B. 2007. V.
851. P. 250-256.
5. Buck R.H., Krummen K. High-performance liquid chromatographic determination of
enantiomeric amino acids and amino alcohols after derivatization with o-phthaldialdehyde
and various chiral mercaptans. // J. Chromatogr. 1987. V. 387. P. 255-265
6. Agilent Zorbax Eclipse AAA instruction for use. Pub. 5980-3088 EN. Agilent
Technologies, 2000
7. Шатц В.Д., Сахартова О.В. Высокоэффективная жидкостная хроматография.
Рига. Зинатне. 1988.
determination of amino acidenantiomers by derivatization with o-phthaldialdehyde and
chiral thiols. Applications with reference to food science // Journal of Chromatography A.
1995. V 697. P 229-245.
2. Чернобровкин М.Г., Шаповалова Е.Н., Шпигун О.А., Ананьева И.А.
Определение энантиомеров аминокислот в фармацевтических препаратах методом
обращенно-фазовой ВЭЖХ // Журн. аналит. химии. 2004. Т.59. №1. С. 64-73.
3. S. L. Grant, Y. Shulmanb, P. Tibbo, D. R. Hampson, G. B. Bakera, Determination of
d-serine and related neuroactive amino acids in human plasma by high-performance liquid
chromatography with fluorimetric detection // J. Chromatogr B. 2006. V. 844. P. 278-282.
4. I. Pérez-Neria, E. Castrob, S. Montesa, M.-C. Bolla, J. Barges-Coll, José Luis Soto-
Hernándezd, Camilo Ríos. Arginine, citrulline and nitrate concentrations in the
cerebrospinal fluid from patients with acute hydrocephalus. // J. Chromatogr B. 2007. V.
851. P. 250-256.
5. Buck R.H., Krummen K. High-performance liquid chromatographic determination of
enantiomeric amino acids and amino alcohols after derivatization with o-phthaldialdehyde
and various chiral mercaptans. // J. Chromatogr. 1987. V. 387. P. 255-265
6. Agilent Zorbax Eclipse AAA instruction for use. Pub. 5980-3088 EN. Agilent
Technologies, 2000
7. Шатц В.Д., Сахартова О.В. Высокоэффективная жидкостная хроматография.
Рига. Зинатне. 1988.
Published
2019-11-27
How to Cite
Natykan, A. A., Chernobrovkin, M. G., & Shpigun, O. A. (2019). Chromatographic behavior of amino acid derivatives obtained with o-phthalic aldehyde and various nucleophilic agents. Sorbtsionnye I Khromatograficheskie Protsessy, 11(6). Retrieved from https://journals.vsu.ru/sorpchrom/article/view/2001
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