Use of the hyperbranched (hypercrosslinked) polystyrene as sorbent for solid-phase extraction at the analysis of drugs (medicines) in biological objects with high performance thin layer chromatography (HPTLC)
Keywords:
HPTLC, analysis of drugs, steroids, solid-phase extraction, hyperbranched polystyrene.
Abstract
Possibility of simultaneous determination hydrophobic and hydrophilic drugs (medical
products), used at treatment of various dysfunctions of adrenal glands, and on real objects (urine and
serum) by method HPTLC with densitometry detection with use off-line concentration on sorbent
hyperbranched polystyrene Purosep-270 and on-line on a plate was shown. The comparative quantitative
estimation liquid and solid-phase extraction on С18, silicagel and hyperbranched polystyrene Purosep-
270 have been done. The increase in selectivity of division has been developed with use of various
modifiers of a stationary phase (β-cyclodecstrene (β-CD) and cationic and anionic surface-active
substances).
Downloads
Download data is not yet available.
References
1. Ларионов О.Г. Руководство по современной тонкослойной хроматографии /
Москва. 1994. 311 С.
2. Akula K.K., Kaur M., Kulkarni S.K. Estimation of adenosine and its major metabolites
in brain tissues of rats using high-performance thin-layer chromatography–densitometry. //
J. Сhromatography A. V. 1209. P. 230-237.
3. Красиков В.Д. Современная планарная хроматография // Журнал аналитической
химии. 2003. Т. 58. №8. С. 792 – 807.
4. Osbourn D.M., Weiss D.J., Lunte G.E. On-line preconcentration methods for capillary
electrophoresis.// Electrophoresis. 2000. V. 21. № 14. P. 2768.
5. Shihabi Z.K. Stacking in capillary zone electrophoresis. // J. Chromatogr. A. 2000. V.
902. № 1. P. 107.
6. Hennion M.-C. Solid-phase extraction: method development, sorbents, and coupling
with liquid chromatography // J. Chrom. 1999. V. 856. P. 3-54.
7. Flood K.G., Reynolds E.R., Snow N.H. Characterization of inclusion complexes of
betamethasone – related steroids with cyclodextrins using high – performance liquid
chromatography // J. Chromatogr. A. 2000. V. 903. P. 49 – 65.
8. Карцова Л. А., Бессонова Е. А., Великанова Л. И., Павлова Е. Г. «Влияние β-
циклодекстрина на факторы удерживания стероидных гормонов ОФ ВЭЖХ» //
Вестник СПбГУ. Сер. 4. 2005. Вып. 1. С. 78 – 84.
9. Цюрупа М.П., Даванков В.А. ССП новый тип сорбентов./ Итоги науки и
техники. Хроматография. 1984. C. 32 – 63.
10. Цюрупа М.П., Панкратов Е.А., Цванкин Д.Я., Жуков В.П., Даванков В.А.
Морфология макросетчатых изопористых полимеров стирола типа «Стиросорб».//
Высокомолекулярные соединения. 1985. Т. XXVII. №2. C. 339 – 345.
11. Davankov V.A., Tsyurupa M.P. Structure and properties of hypercross-linked
polystyrene the first representive of a new class of polymer networks React. Polymer.
1990. V. 13. P. 27-42.
12. Pastukhov A.V., Tsyurupa M.P., Davankov V.A. Hypercrosslinked Polystyrene: A
Polymer in a Non-Classical Physical State. // J. Polymer Science: Part B: Polymer Physics.
1999. V. 37. P. 2324 – 2333.
13. Davankov V.A., Sychov C.S., Ilyin M.M., Sochilina K.O. Hypercrosslinked
polystyrene as a novel type of high-performance liquid chromatography column packing
material. Mechanisms of retention // J. Chromatography A. 2003. V. 987. P. 67-75.
Москва. 1994. 311 С.
2. Akula K.K., Kaur M., Kulkarni S.K. Estimation of adenosine and its major metabolites
in brain tissues of rats using high-performance thin-layer chromatography–densitometry. //
J. Сhromatography A. V. 1209. P. 230-237.
3. Красиков В.Д. Современная планарная хроматография // Журнал аналитической
химии. 2003. Т. 58. №8. С. 792 – 807.
4. Osbourn D.M., Weiss D.J., Lunte G.E. On-line preconcentration methods for capillary
electrophoresis.// Electrophoresis. 2000. V. 21. № 14. P. 2768.
5. Shihabi Z.K. Stacking in capillary zone electrophoresis. // J. Chromatogr. A. 2000. V.
902. № 1. P. 107.
6. Hennion M.-C. Solid-phase extraction: method development, sorbents, and coupling
with liquid chromatography // J. Chrom. 1999. V. 856. P. 3-54.
7. Flood K.G., Reynolds E.R., Snow N.H. Characterization of inclusion complexes of
betamethasone – related steroids with cyclodextrins using high – performance liquid
chromatography // J. Chromatogr. A. 2000. V. 903. P. 49 – 65.
8. Карцова Л. А., Бессонова Е. А., Великанова Л. И., Павлова Е. Г. «Влияние β-
циклодекстрина на факторы удерживания стероидных гормонов ОФ ВЭЖХ» //
Вестник СПбГУ. Сер. 4. 2005. Вып. 1. С. 78 – 84.
9. Цюрупа М.П., Даванков В.А. ССП новый тип сорбентов./ Итоги науки и
техники. Хроматография. 1984. C. 32 – 63.
10. Цюрупа М.П., Панкратов Е.А., Цванкин Д.Я., Жуков В.П., Даванков В.А.
Морфология макросетчатых изопористых полимеров стирола типа «Стиросорб».//
Высокомолекулярные соединения. 1985. Т. XXVII. №2. C. 339 – 345.
11. Davankov V.A., Tsyurupa M.P. Structure and properties of hypercross-linked
polystyrene the first representive of a new class of polymer networks React. Polymer.
1990. V. 13. P. 27-42.
12. Pastukhov A.V., Tsyurupa M.P., Davankov V.A. Hypercrosslinked Polystyrene: A
Polymer in a Non-Classical Physical State. // J. Polymer Science: Part B: Polymer Physics.
1999. V. 37. P. 2324 – 2333.
13. Davankov V.A., Sychov C.S., Ilyin M.M., Sochilina K.O. Hypercrosslinked
polystyrene as a novel type of high-performance liquid chromatography column packing
material. Mechanisms of retention // J. Chromatography A. 2003. V. 987. P. 67-75.
Published
2019-11-27
How to Cite
Kartsova, L. A., Davankov, V. A., Bessonova, E. A., & Obyedkova, E. V. (2019). Use of the hyperbranched (hypercrosslinked) polystyrene as sorbent for solid-phase extraction at the analysis of drugs (medicines) in biological objects with high performance thin layer chromatography (HPTLC). Sorbtsionnye I Khromatograficheskie Protsessy, 10(1). Retrieved from https://journals.vsu.ru/sorpchrom/article/view/2015
Section
Статьи
