Upscaling of protein frontal chromatography processes on carboxylic monolithic media
Keywords:
Adsorption, Ion-exchangers, Proteins, Monoliths, Chromatography.
Abstract
Upscaling of frontal chromatographic processes using the monolithic 8 ml column on basis of
glycidylmethacrylate-ethylenglycoldimethacrylate (GMA-EDMA) copolymer was performed. By means
of carboxyimethylation of the polymer surface the cation-exchanger with the total exchange capacity 0.2
mg-equiv/g was obtained. Experiments with dynamic sorption of the model cytochrom c - BSA or
cytochrom c – fibrinogen mixtures showed the increase of the sorption selectivity for the larger proteins
in comparison to the monolithic 0.3 ml disks. Results obtained for the model mixtures were used in the
process of plasma proteins separation.
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Applications. // Elsevier. - 2003. P. 773.
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4. Joisic D., Ceifton J. Use of monololitic supports in proteomics technology // J.
Chromatogr. A -2007. V.1144. Iss.1. P.2-13.
5. Очкур О.В., Демин А.А., Тенникова Т.Б. Избирательность сорбции белков на
карбоксильных катионитах различного строения // Сорбционные и
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цитохрома С на карбоксильном катионите КМДМ-6-5 // ЖФХ – 1999. Т. 73. №11.
С. 2068-2071.
9. Laemmli, UK Cleavage of structural proteins during the assembly of the head of
bacteriophage T4 // Nature. 1970. V.227. P. 680-685.
10. Папукова К.П., Никифорова Е.С., Демин А.А. и др. Гидрофильные
гетеросетчатые полиэлектролиты с варьируемым содержанием карбоксильных групп
// Высокомолекулярные соединения. - 2004. T. А46. №8. C. 1-5.
11. Demin A.A., Melenevsky A.T., Papukova K.P.. The effect of the concentration of
ionogenic groups of the sorbent on the separation of protein mixtures ⁄⁄ J.Chromatogr. A. -
2007. V.1006. P.185.
12. Demin A.A., Ochkur O.V. Cation exchangers for selective sorption of large proteins.
⁄⁄ Journal of Chromatogr. B. 2007. V.849, Iss. 1-2. P. 231-235.
Published
2019-11-27
How to Cite
Demin, A. A., Ochkur, O. V., Melenevsky, A. T., Pavlova, E. N., & Nikiforova, E. S. (2019). Upscaling of protein frontal chromatography processes on carboxylic monolithic media. Sorbtsionnye I Khromatograficheskie Protsessy, 10(1). Retrieved from https://journals.vsu.ru/sorpchrom/article/view/2016
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