High performance liquid chromatography determination of the major amino acids in complex biological objects using phenylisothiocyanate derivatization
Keywords:
High performance liquid chromatography, amino acids, pancreas and prostata glands, mixed fodder, a pancreas extract, meat, phenylisothiocyanate.
Abstract
The technique of determination of the major amino acids in complex biological matrixes
(pancreas and prostata glands, mixed fodders and samples of meat) by reversed-phase HPLC with UVdetection
is suggested. The method includes samples hydrolysis with hydrochloric acid and derivatization
of amino acids using phenylisothiocyanate solution in isopropanol. Influence of mobile phase рН on
resolution of amino acids is established. Conditions of acid hydrolysis at stage of sample preparation are
optimised.
Downloads
Download data is not yet available.
References
1.Садек П. Растворители для ВЭЖХ, М.: «Бином», 2006. 703 c.
2.Introduction to HPLC, Shimadzu, Japane, 2008
3.Noctor G., Foyer C. Simultaneous Measurement of Foliar Glutathione,
Glutamylcysteine, and Amino Acids by High-Performance Liquid Chromatography:
Comparison with Two Other Assay Methods for Glutathione // J. Anal. Biochem. 1998. V.
264. P. 98.
4.Lunte S. M., Mohabbat T., Wong O.S., Kuwana T. Determination of desmosine,
isodesmosine, and other amino acids by liquid chromatography with electrochemical
detection following precolumn derivatization with naphthalenedialdehyde/cyanide // J.
Anal. Biochem. 1989. V. 202. P. 178.
5.Буланова А.В., Полякова Ю.Л. Хроматография в медицине и биологии, Самара:
2006. 105 с.
6.Teerlink T., van Leeuwan P.A.M., Houdijk A.// J. Clin. Chem. 1994. V. 245. P. 40.
7.Государственная фармакопея СССР, Т. 2. М.: «Медицина», 1990. 397 с.
8.Fujimura K., Suzuki S., Hayashi K., Masuda S.// J. Anal. Chem. 1990. V. 198. P. 62.
9.Poboży E., Czarkowska W., Trojanowicz M. Determination of amino acids in saliva
using capillary electrophoresis with fluorimetric detection // J. Biochem. and Biophys.
Meth. 2006. V. 67. P. 37-47.
2.Introduction to HPLC, Shimadzu, Japane, 2008
3.Noctor G., Foyer C. Simultaneous Measurement of Foliar Glutathione,
Glutamylcysteine, and Amino Acids by High-Performance Liquid Chromatography:
Comparison with Two Other Assay Methods for Glutathione // J. Anal. Biochem. 1998. V.
264. P. 98.
4.Lunte S. M., Mohabbat T., Wong O.S., Kuwana T. Determination of desmosine,
isodesmosine, and other amino acids by liquid chromatography with electrochemical
detection following precolumn derivatization with naphthalenedialdehyde/cyanide // J.
Anal. Biochem. 1989. V. 202. P. 178.
5.Буланова А.В., Полякова Ю.Л. Хроматография в медицине и биологии, Самара:
2006. 105 с.
6.Teerlink T., van Leeuwan P.A.M., Houdijk A.// J. Clin. Chem. 1994. V. 245. P. 40.
7.Государственная фармакопея СССР, Т. 2. М.: «Медицина», 1990. 397 с.
8.Fujimura K., Suzuki S., Hayashi K., Masuda S.// J. Anal. Chem. 1990. V. 198. P. 62.
9.Poboży E., Czarkowska W., Trojanowicz M. Determination of amino acids in saliva
using capillary electrophoresis with fluorimetric detection // J. Biochem. and Biophys.
Meth. 2006. V. 67. P. 37-47.
Published
2019-11-27
How to Cite
Rudenko, A. O., Kartsova, L. A., & Snarskiy, S. I. (2019). High performance liquid chromatography determination of the major amino acids in complex biological objects using phenylisothiocyanate derivatization. Sorbtsionnye I Khromatograficheskie Protsessy, 10(2). Retrieved from https://journals.vsu.ru/sorpchrom/article/view/2041
Section
Статьи
