Separation of fat-soluble vitamins D2 and E at the joint presence of TLC
Keywords:
tocopherol acetate, ergocalciferol, thin layer chromatography, fatty oils.
Abstract
The work is dedicated to the development of methods for determining and division of fat-soluble
vitamins D2 and E by the method of TLC when present together. A study of the different elution systems,
constructed according to Rf values tocopherol acetate and ergocalciferol polarity eluent value intervals
defined polarity eluent in which the data are linear function. Based on the identified dependencies, the
possibility of a theoretical approach to the selection of the optimal conditions of chromatographic separation
of these substances in a thin layer at the joint presence. The developed method was tested on vegetable oil sea
buckthorn fruit. The technique can be used in the quality control of vegetable oils and oil extracts of
pharmaceutical and complex multivitamin preparations.
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tekhnika», 1979, pp. 80-129.
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khromatografiya, M.: «Mir», 1981, pp. 402-
407.
3. Sharshunova M., Shvarts V., Mikhalets Ch.
Tonkosloinaya khromatografiya v farmatsii i
klinicheskoi biokhimii, M.: «Mir», 1980, V. 2,
610 p.
4. ND 42-7843-97. «Vitamin E v kapsulakh»
5. Luttseva A.I., Maslov L.G., Seredenko V.I.
Metody kontrolya i standartizatsii lekarstvennykh
preparatov, soderzhashchikh zhirorastvorimye
vitamin [Methods of control and
standardization of medicinal products
containing fat-soluble vitamins], Pharmaceutical
Chemistry Journal, 2001, V. 35, No. 10, pp. 41-
45.
6. FSP 42-0008018000 «Ergokal'tsiferol
rastvor v masle 0,5 %».
7. Kozlov E. I., Solunina I.A., Lyubareva M.
L. et al. Opredelenie vitaminov A, D, E v
polivitaminnykh preparatakh s pomoshch'yu
vysokoeffektivnoi zhidkostnoi khromatografii
[Determination of vitamins A, D, E in
multivitamin preparations using HPLC],
Pharmaceutical Chemistry Journal, 2003, V. 37,
No 10, pp. 50-53.
8. Klyuev S.A. Opredelenie vitaminov A i E
metodom VEZhKh s predvaritel'nym
ravnovesnym raspredeleniem v dvukh
nesmeshivayushchikhsya zhidkikh faza
[Determination of vitamins A and E by HPLC
with pre-equilibrium distribution in two
immiscible liquid phase], Journal of Analytical
Chemistry, 1996, V. 51, No 9, pp. 961-963.
9. Denisova L.V., Filimonov V.N.,
Balyatinskaya L.N. et al. Khromatograficheskoe
povedenie zhirorastvorimykh vitaminov v
rezhime obrashchennoi-fazovoi zhidkostnoi
khromatografii s podvizhnoi fazoi voda –
izopropanol [Chromatographic behavior of fatsoluble
vitamins in the mode of reverse phase
liquid chromatography under the vision-phase
water – isopropanol], Journal of Analytical
Chemistry, 1997, V. 52, No 9, pp. 967-969.
10. Gosudarstvennaya farmakopeya X izd.,
M.: Meditsina, 1968, pp. 627-628; 707-708.
11. Gosudarstvennaya farmakopeya XI izd.,
M.: Meditsina, 1990, No 2, С. 41-59.
12. Gosudarstvennaya farmakopeya
Rossiiskoi Federatsii XII izd. – Chast' 1, M.:
Izd-vo: Nauchnyi tsentr ekspertizy sredstv
meditsinskogo naznacheniya, 2008, 704 p.
13. Rudakov O.B., Vostrov I.A., Fedorov
S.V. et al. Sputnik khromatografista. Metody
zhidkostnoi khromatografii, Voronezh: Izd-vo
«Vodolei», 2004, 528 p.
14. Geiss F. Osnovy tonkosloinoi
khromatografii, M.: Mir, 1999, 405 p.
15. Checheta O.V., Safonova E.F., Slivkin
A.I. Izuchenie khromatograficheskikh
kharakteristik β-karotina v tonkom sloe sorbenta
[The study of the chromatographic
characteristics β-carotene in a thin layer of
sorbent], Pharmaceutical Chemistry Journal,
2012, V. 46, No 5, pp. 54-56.
16. Borodina E.V., Kitaeva T.A., Safonova
E.F. et al. Opredelenie α-tokoferola i
ergokal'tsiferola metodom tonkosloinoi
khromatografii [Determination of α-tocopherol
and ergocalciferol by Thin-Layer
Chromatography], Journal of Analytical
Chemistry, 2007, V. 62, No 11, pp. 1181-1185.
Published
2018-02-16
How to Cite
Тринеева, О. В., Сафонова, Е. Ф., & Сливкин, А. И. (2018). Separation of fat-soluble vitamins D2 and E at the joint presence of TLC. Sorbtsionnye I Khromatograficheskie Protsessy, 15(1), 42-50. https://doi.org/10.17308/sorpchrom.2015.15/253
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