Electrochemical synthesis and sorption characteristics of polymers with molecular imprints of clenbuterol and ractopamine
Abstract
The method of cyclic voltammetry with linear potential sweep within the range of -0.2-0.8 V was used to study the conditions of electrochemical synthesis on the surface of the sensor electrode of a high-affinity thin film coating based on a polymer with molecular imprints of clenbuterol and ractopamine. The electro-chemical synthesis of the MIP was carried out in a three-electrode cell: the working electrode was a piezoelec-tric resonator of AT-cut, the auxiliary electrode were stainless steel plates, and the reference electrode was a silver chloride electrode. We described the conditions for the production of MIP films based on methylene blue or pyrrole, a copolymer of pyrrole and aniline, as well as pyrrole and disintegrating reagents, hydroquinone, pyrocatechol, and resorcinol. To assess the quality of the electro-synthesized coating, surface topography was monitored (atomic force microscopy method), as well as the change in the mass of the MIP film during its formation and after the removal and re-incorporation of template molecules (the method of piezoelectric quartz micro-weighing). It was established that the formation of stable polymer films based on polypyrrole or polypyr-role with hydroquinone occurs within one polymerisation cycle, polypyrrole and aniline occurs within two cycles, and methylene blue occurs within 60 polymerisation cycles. It was shown that the production of clen-buterol and ractopamine MIP films based on pyrrole and hydroquinone or methylene blue results in the for-mation of structures with a high concentration of stable molecular imprints.
The analytical signal of the sensor was recorded against the sensor with a non-imprinted polymer (NIP) to exclude the contribution of non-specific interactions to the signal on the surface of the sensor. Sensors based on electro-synthesised MIP layers were tested in the analysis of model solutions of clenbuterol and meat samples. Graduation graphs are linear in the range of 7.5-30 μg/ml (for sensors based on polypyrrole and hy-droquinone) and 11.3-60.0 μg/ml (for sensors based on methylene blue), the detection limit of clenbuterol is (μg/ml) 5.6 and 0.46, respectively.
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References
Crapnell R.D., Hudson A., Foster C.W., Eersels K. et al., Sensors, 2019, Vol. 19, pp. 1204-1232.; doi.org/10.3390/s19051204
Cosnier S., Electroanalysis, 2005, Vol. 17, No 19, pp. 1701-712. doi.org/10.1002/elan.200503308
Li G., Wang Y., Xu H., Sensors, 2007, Vol. 7, pp. 239-250.
Feng L., Liu Y., Tan Y., Hu J., Biosens. Bio-electron., 2004, Vol. 19, No 11, pp. 1513-1519.
Avila M., Zougagh A., Escarpa A., Trends in Anal. Chem., 2008, Vol. 27, No 1, pp. 54-65.
Karaseva NA., Soboleva I.G., Ermolaeva T.N., Sorbtsionnye i khromatograficheskie protsessy, 2013, Vol. 13, No 1, pp. 5-9.
Ermolaeva T.N., Chernyshova V.N., Chesnokova E.V., Bessonov O.I., Sorbtsionnye i khromatograficheskie protscessy, 2015, Vol. 15, No 2, pp. 151-167.
Ermolaeva T.N., Farafonova O. V., Bes-sonov O.I., Sorbtsionnye i khromatograficheskie protsessy, 2019, Vol. 19, No 6, pp. 682-690.
Karaseva N., Ermolaeva T., Mizaikoff B., Sens. Actuators B, 2016, Vol. 225, pp. 199-208.
Ermolayeva T.N., Farafonova O.V., Bes-sonov O.I., J Anal. Chem., 2019, Vol. 74, No 2, pp. 1-8.
Khalkhali R.A., Electrokhimiya, 2005, Vol. 41, No 9, pp. 1071-1078.
Ebarvia B.S., Cabanilla S., Sevilla F., Ta-lanta, 2005, Vol. 66, No 1, pp.145-152.
Maouchea N., Guergouri M., Gam-Der-ouich S., Jouini M. et al., J. Electroanal. Chem., 2012, Vol. 685, pp. 21-27.
Karyakin A.A., Strakhova A.K., Karyakina E.E., Varfolomeyev S.D. et al., Synthetic Met-als., 1993, Vol. 60, pp. 289-291.
Liu J., Mu Sh., Synthetic Metals., 1999, Vol. 107, pp 159-160.
