Studying the molecular weight distribution of chitosan obtained from the mycelium of Aspergillus niger fungus by means of gel chromatography
Abstract
The article presents the results of the study of molecular chitosan obtained from the mycelium of the Aspergillus niger fungus as compared to a sample produced by Fratelli Parodi (control sample). Chitosan is a copolymer of glucosamine and N-acetylglucosamin and a polymolecular biopolymer. The applications of chitosan are largely determined by its sorption and biological activity, which depends a lot on the molecular mass. The aim of our study was to investigate the molecular weight distribution (MWD) of the polymer mol-ecules of chitosan using gel permeation chromatography.
The obtained chromatograms of the output of the polymer molecules of the studied chitosan samples demonstrated 3 characteristic peaks, corresponding to the three main fractions: a high molecular weight frac-tion (ММ 34-105 kDa), an average molecular weight fraction (ММ 17.5-27.5 kDa) and a low molecular weight fraction (ММ 4.5-13.5 kDa). The study determined the molecular weight composition, the weight- and number-average weight of certain chitosan fractions.
The MWD curves characterising the molecular weight distribution among fractions were obtained. The analysis of the curves demonstrated that the sample obtained from the mycelium of the Aspergillus niger fungus and the control sample have relatively identical polymolecular composition. The largest fraction is the high molecular weight fraction (about 60%). The mass fraction of the average molecular weight fraction in the control chitosan sample is 20%, while in the studied sample it is 10%. The portion of the low molecular weight fraction is 20% in the control sample and 30% in the studied sample. It is demonstrated that the dis-persion of molecular weights is close to 1, which is characteristic of monomolecular biopolymers.
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