Газохроматографическое изучение состава экзополисахаридов и их роли в защите от оксидативного стресса у микроаэрофильных бактерий «Thioflexothrix psekupsii» D3 gen. nov., sp. nov.

Ю. П. Федоненко; М. В. Орлова; С. С. Евстигнеева; Е. В. Белоусова; М. Ю. Грабович

doi:10.17308/sorpchrom.2015.15/309

Ю. П. Федоненко Fedonenko Yulia P. – PhD in Microbiology and Biochemistry, Senior Researcher in the Laboratory of Biochemistry, Institute of Biochemistry and Physiology of Plants and Microorganisms RAS, Saratov
М. В. Орлова Voronezh State Univercity, Voronezh, e-mail: maryorl@mail.ru
С. С. Евстигнеева Yevstigneyeva Stella S. – PhD student in the Laboratory of Biochemistry, Institute of Biochemistry and Physiology of Plants and Microorganisms RAS, Saratov
Е. В. Белоусова Belousova Elena Vasilevna – PhD, assistant, Voronezh State University, the Biology Department, Division of Cell Biochemistry and Physiology, Voronezh
М. Ю. Грабович Grabovich Margarita Yurievna - professor in the Department of Cell Biochemistry and Physiology, Voronezh State University, Voronezh, e-mail: margarita_grabov@mail.ru

DOI: https://doi.org/10.17308/sorpchrom.2015.15/309

Keywords: exopolysaccharides (EPS), gas-liquid chromatography, Thioflexothrix psekupsii.

Abstract

The aim of this work was to study the composition of exopolysaccharides at the new representative
of colorless sulfur bacteria «T. psekupsii», and to proof their participation in the antioxidant protection.
The composition of «T. psekupsii» exopolysaccharides was analyzed by GLC. It was established
that the main component of «T. psekupsii» EPS was galactose and firmly bound to the cell wall EPS fraction
was presented by high molecular galactose homopolymer. Chromatographic data were confirmed by genome
sequencie, which revealed the presence of genes that determine the enzymes involved into the synthesis of
galactose and galactan: galE, encoding UDP-glucose 4-epimerase (EC 5.1.3.2), glF, encoding UDPgalactopyranose
mutase (EC 5.4.99.9).
EPS concentration was determined by the phenol method at different oxygen concentrations to
prove its role in the antioxidant protection. The correlation between O2 concentration in the cultural medium
and EPS concentration was revealed. With the decreasing of O2 concentration the concentration of EPS
decreases too. It is shown that «T. psekupsii» spends ten times more carbon for EPS synthesis than for
protein synthesis, which indicates the importance of EPS for this bacterium.
These data demonstrate that «T. psekupsii» EPS are presented by the galactose polymer, which is
involved into the cell protecting from the damaging effects of oxygen.

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Gas chromatographic study of exopolysaccharides composition and their role in the protection against oxidative stress in microaerophilic bacterium «Thioflexothrix psekupsii» D3 gen. nov., sp. nov.

Abstract

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