Study of complexation and stability in inter ligand exchange reactions of N-vinylamide co-polymers with gallium and indium by liquid chromatography.
Abstract
At present time nuclear medicine is rapidly developing field of medicine which allows to obtain
high-resolution quantitative data on the pathologies of the living organism and to monitor physiological and
biochemical. The goal of this work is to develop methods of study of the complexation of biologically active
copolymer systems with ions of trivalent metals (indium, gallium) by liquid chromatography for
development of novel macromolecular radiopharmaceuticals. Liquid chromatography is essentially the single
method of determining of radiochemical yield and purity of the radiopharmaceuticals, and tool for isolation
and purification of preparation.
HPLC was used in two modifications: a monolithic chromatography on ultra short monolith
columns for evaluation of kinetics of complex formation and the traditional size-exclusion chromatography
mode for determination of molecular weight characteristics of the metal-polymer complexes (MPC) and for
study histidine challenge reaction. It was shown that the complex formation with chelation units
(iminodiacetic acid) of copolymer of vinylpyrrolidone and vinyl formamide with a gallium is more reactive
than that of indium. The obtained MPC are stable at physiological pH for long time. Reaction of Ga-MPC
with histidine appeared to be fairly stable in contrast to In-MPC analogue, which was completely
decomposed in the same conditions.
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