The study of probe loading capacity of HPLC monolithic columns based on organic porous polymers
Abstract
The loading properties of HPLC monolithic columns based on 1-vinylimidazole and 4-vinylpyridine
were studied. These properties were compared with commercial packed column (Diasfer-250-ST) of the
same dimension based on divinylbenzene-styrene polymer.
It was shown that for dried monolith sorbents the textural characteristics (specific surface area, pore
volume and dimensions) can’t be used as a sample loading indicator. This is connected with a porous system
modification in the organic monolith placed in a «good» solvent. Diasfer column packaging material is not
affected by the solvent.
Therefore, proper selection of the mobile phase composition for investigated monolithic columns allow
to work with the large samples about 100 µg. This is significantly exceeds the quantity of the sample size
to a Diasfer packed column (30-40 µg).
Downloads
References
2. Tennikova T.B., Bleha M., Svec F., J. Chromatogr. A, 1991, Vol. 555, pp. 97-107.
3. Svec F., Fréchet J., Anal. Chem., 1992, Vol. 64, No 7, pp. 820-822.
4. Wang Q.C., Svec F., Fréchet J.M.J., Anal. Chem., 1993, Vol. 65, No 17, pp. 2243-2248.
5. Kubin M., Spacek P., Chromecek R, Coll. Czechosl. Chem. Commun., 1967, Vol. 32, pp. 3881-3887.
6. Guiochon G., J. Chromatography A, 2007, Vol. 1168, pp. 101-168.
7. Davies N.H., Euerby M.R., McCalley D.V., J. Chromatogr. A, 2007, Vol. 1138, pp. 65-72.
8. Davies N.H., Euerby M.R., McCalley D.V., J. Chromatogr. A, 2006, Vol. 1119, pp. 11-19.
9. Eble J.E., Grob, R.L., Antle P.E., Snyder L.R., J. Chromatogr. A, 1987, Vol. 405, pp. 31-60.
10. Gray M.J., Slonecker P.J., Dennis G., Shalliker R.A., J. Chromatogr. A, 2005, Vol. 1096, pp. 92-100.
11. Laher M., Causon T.J., Buchberger W., Hild S. et al., Anal. Chem., 2013, Vol. 85. No 12, pp. 5645-5649.
12. Koeck R., Fischnaller M., Bakry R., Tessadri R. et al., Anal. Bioanal. Chem., 2014, Vol. 406, No 24, pp. 5897-5907.
13. Mohr J.H., Swart R., Huber C.G., Anal. Bioanal. Chem, 2011, Vol. 400, No 8, pp. 2391-2402.
14. Smirnov K.N., Dyatchkov I.A., Pirogov A.V., Shpigun O.A., Moscow Univ. Chem. Bull., 2011, Vol. 66, No 6, pp. 351-355.
15. Gritti F., Guiochon G, J. Chromatogr. A,. 2014, Vol. 1362, pp. 49-61.
16. Nevejans F., Verzele M.,J. Chromatogr. A, 1985, Vol. 350, pp. 145-150.
17. Yudina Y.S. Patrushev Y.V., "Monolithic HPLC columns for nitrogen-based heterocyclic compounds", Proceedings of the X Russian Scientific Conference with international participation. 12-17 september 2016, Barnaul, 2016, pp. 153.
18. Gusev I., Huang X., Horváth C., Journal of Chromatography A, 1999, Vol. 855, pp. 273-290.
19. Sing K.S.W., Pure Appl.Chem., 1982, Vol. 54, No 11, pp. 2210.
