Determination of products of reactions adenosine with α-hydroxyethyl radical by HPLC MS

Abstract

The free radicals are the one of the reasons of the variety diseases. Because of rising grade of the
concertation of the free radicals are correlating with strong diseases as cancer, atherosclerosis and others. In
our times the main interest is caused by oxygen reactions and nitrogen-centered radicals. In spite of this fact,
all cases of the reaction of these radicals have with them a formation of carbon-centered radicals. This formation
is registered in vitro.
It is well known fact, that the reactions of the hydroxyl radicals with DNA may produce a formation
of covalent cross-links between DNA and lipid with DNA-DNA. Carbon-centered radicals are able to join in
the nucleotide molecule at the double bonds. Therefore, we observe formation cross-links which are hardly
repairable damages.
The aim of the research is to studying of the product of the hydroxyl radicals reactions with the molecule
adenosine. The plan of the work on the interactions adenosine with hydroxyethyl radicals is: the extirpation
of oxygen out of the solution, radiolysis of hydro-ethanolic solutions (the ethanol concentration is
5%, HCl is 0.1%, nucleoside 10-3 mol/l). The identification of the x-ray the products induced transformations
performed by nucleoside by UPLC MS by «Acquity UPLC» with mass-detector TQD”.
The chemical species lose neutral fragment ( m/z 132) which is the ribose. It was identified.
It means that changes are made in base, but not in the carbohydrate branch. Because of the increasing mass,
are discovered that the main interaction process is the connection hydroxyethyl radical with C8. In prospect,
the first adduct interacts with the reactions of the recombination, which are disproportion of two adducts,
simple dimerization of adduct with the hydroxyethyl radical and the dimerization with the elimination of the
hydroxyl group.
In research work, was revealed the products of interaction adenosine with carbon-centered radical by
reverse-phase HPLC with DAD and MS-detector. It was established that basic processes of interactions was
addition hydroxyethyl radical in C8 position. The products of recombination hydroxyethyl radicals with ini-tial adducts was been detected. Based on the results of the research conducted, has been shown of research of
possibility cross-linker formation in DNA under the influence of free-radicals