Immobilization of doxycycline hydrochloride on clinoptilolite
Abstract
The use of enterosorbents based on aluminosilicates as drug carriers to increase the stability and effectiveness of their use is considered promising in modern pharmacological studies for the immobilization of drugs on natural sorbents. One of the representatives of such aluminosilicates is clinoptilolite, a zeolitic tuff with a developed surface, high sorption capacity and the possibility of modification. From the group of semi-synthetic tetracycline antibiotics, doxycycline hydrochloride, compared with other tetracyclines, practically does not inhibit the normal intestinal microflora and it is characterized by high bioavailablity. The purpose of this study was the investigation of the sorption capacity of clinoptilolite with respect to doxycycline hydrochloride.
"Klimont" - clinoptilolite tuff of the Lyulinskoe deposit (Polar Urals of Yugra), the main phase of which is clinoptilolite (68%), and an antibiotic - doxycycline hydrochloride was recommended as an enterosorbent.
Sorption equilibrium in the clinoptilolite - aqueous solution of doxycycline hydrochloride system was studied at a temperature of 298 K under static conditions by the method of variable concentrations in the range 0.05-4.0 mmol/dm3 (pH=4.5). The filtrate was analysed for antibiotic content by a spectrophotometric method using a Shimadzu UV-1800 spectrophotometer. The flame photometry and complexometry methods were used for the determination of the content of extra framework cations of the sorbent.
The sorption isotherm of doxycycline hydrochloride on clinoptilolite was obtained. The contribution of the exchange and non-exchange components to the sorption parameter is determined. It was established that the mechanism of the equivalent exchange of extra framework cations of the sorbent for cations of the preparation due to the electrostatic interaction between positively charged NH3+ groups of doxycycline and electronegative centres of the sorbent framework corresponds to the monomolecular sorption of an antibiotic on aluminosilicate. It was found that the formation of polymolecular layers is possible as a result of the association of doxycycline due to hydrogen bonds of the C=O groups of the pyranose ring and hydroxyl groups of phenol groups.
The sorption isotherm of doxycycline hydrochloride on clinoptilolite was processed using the Langmuir, Freundlich and Redlich-Peterson models. It was found that antibiotic sorption from dilute solutions is best described by the Langmuir model. Values of sorption parameters using linearised equations of sorption models and equilibrium distribution coefficient were calculated.
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Kevadiya B.D., Joshi G.V., Patel H.A., Ingole P.G., Mody H.M., Bajai H.C. Montmorillonite–alginate nanocomposites as a drug delivery system: Intercalation and in vitro release of vitamin B1 and vitamin B6. Journal of Biomaterials Aplications. 2010; 25(2): 161-177. https:/doi.org/10.1177/0885328209344003 (In Russ.).
Farıas T., Ruiz-Salvador A.R., Ve-lazco L., Menorval L.D., Rivera A. Prepara-tion of natural zeolitic supports for potential biomedical applications. Materials Chemistry and Physics. 2009; 118: 322-328. https://doi.org/10.1016/J.matchemphys.2009.07.054
Chernova R.K., Venig S.B., Naumova G.N. Sorption of tetracycline and its degradation products by glauconite. Scientific Almanac. 2015; (9): 930-934. (In Russ.).
Vlasova N.N. Interaction of highly dispersed silica with some medicinal substances. Surface. 2016; 8(23): 236-247. (In Russ.).
Physico-chemical and medico-biological properties of natural zeolites / ed. by Z.V. Belousov. Novosibirsk: Publishing House of the University of Geology and Geophysics. 1990. 70 p. (In Russ.).
Zhuchkov A.N., Berlyand A.S., Alikhanyan A.S., Plesskaya N.A. Sorption properties of the new natural enterosorbent klimont. Pharm. Chemistry journal. 2011; 45(2): 110-113. https://doi.org/10.1007/s11094-011-0571-5
Egorov N.S. Fundamentals of the doctrine of antibiotics. M. Nauka. 2004. 528 p. (In Russ.).
Nawashin S.M., Fomina I.P. Antibi-otic locator. M. Medicine. 1974. 416 р. (In Russ.).
Kotova D.L., Krysanova T.A., Vasi-lyeva S.Yu., Selemenev V.F., Artamonova M.N. “Analytics of Siberia and the Far East”, materials of the X All-Russian Scien-tific Conference with international partici-pation. September 12-17. 2016. Barnaul. 106 p. (In Russ.).
Polyansky N.G., Gorbunov V.G., Polyanskaya N.L. Methods of ionite re-search. M. Chemistry. 1976. 208 p. (In Russ.).
Nakanishi K. Infrared spectroscopy and the structure of organic compounds. M. Mir. 1965. 216 p. (In Russ.).
Becker Yu. Spectroscopy. M. Tech-nosphere. 2009. 528 p. (In Russ.).
Sing K.S.W. Reporting Physisorp-tion Data for gas/solid Systems with Special Reference to the Determination of Surface Area and Porosity. Pure and Applied Chem-istry. 1982; 57(11): 2201-2218. https://doi.org/10.1351/PAC 19825411221
Kotova D.L., Pham Thi Gam, Krysanova T.A., Vasileva S.Yu., Тimchen-ko Yu.A., Beketov B.N. Description of the isotherm of sorption of pyridoxine hydro-chloride on clinoptilolite tuff. Sorbtsionnye I khromatograficheskiye protsessy. 2014; 14(4): 572-577. (In Russ.).
Langmuir I. The Constitution and Fundamental Properties of Solids and Liquids / I. Langmuir. J. Am. Chem. Soc. 1917; 39(9): 1848-1906. https://doi.org/10.1021/ja02254a006
Freundlich H.M.F. Over the Adsorption in Solution. J. Phys. Chem. 1906; 57: 385-447.
Redlich O.A., Peterson D.L. Useful Adsorption Isotherm. J. Phys. Chem. 1959; 63(6): 1024. https://doi.org/10.1021/j150576a611
Pyulman B. Intermolecular interac-tions: from diatomic molecules to biopoly-mers. M. Mir. 1981. 580 p. (In Russ.).
