Study of dopamine sorption on γ-Al2O3 and Strata-SDBL sorbents
Abstract
Catecholamines, especially dopamine, play an important role in regulating many vital processes in the human body (psychomotor function, cardiovascular function, and mental state). Monitoring their levels in the body allows early detection of certain diseases. The analysis of catecholamines is complicated by their low content in bodily fluids (the dopamine norm for adults is less than 0.13 nM in blood and from 0.3 to 3 µM in daily urine). To determine catecholamines, it is necessary to preconcentrate them. The aim of this work was to study the adsorption of a representative of catecholamine class, dopamine, on sorbents of different nature, to determine the physical and chemical characteristics of its adsorption, and to choose the optimal sorbent for the sorption concentration of catecholamines.
We compared the features of dopamine extraction from aqueous solutions by γ-Al2O3 and Strata-SDBL sorbents under static conditions and studied the effect of various parameters (pH, phase contact time, dopamine concentration) on the sorption. The distribution of dopamine in the water-sorbent system was monitored using RP-HPLC with an amperometric detector. Maximum extraction of dopamine was achieved at pH 8-9 on γ-Al2O3 and at pH 5-11 on Strata-SDBL with sorption time of 20 minutes. Isotherms of dopamine adsorption on the above sorbents were obtained. It was shown that they belonged to the class of Langmuir isotherms. It was determined that γ-Al2O3 had a significantly higher sorption capacity with respect to dopamine and a higher adsorption constant than Strata-SDBL. It was suggested that sorption of dopamine on γ-Al2O3 occurred mainly due to electrostatic interactions and hydrogen bonds, while for Strata-SDBL, it was due to hydrophobic and π-π-interactions.
For the sorption concentration of catecholamines at low concentrations in the sample, it is advisable to use γ-Al2O3. However, Strata-SDBL can be recommended for the express concentration of catecholamines under dynamic conditions.
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References
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