Определение некоторых гипотензивных лекарственных веществ в плазме крови методом высокоэффективной жидкостной хроматографии / тандемной масс-спектрометрии при анализе острых отравлений
Ключевые слова:
ВЭЖХ-МС/МС, атенолол, бисопролол, верапамил, метопролол, нифедипин, пропранолол, эналаприл, острые отравления
Аннотация
Разработана селективная, чувствительная и воспроизводимая методика количественного
определения атенолола, бисопролола, верапамила, метопролола, нифедипина, пропранолола и
эналаприла в плазме крови человека методом обращенно-фазовой высокоэффективной жидкостной
хроматографии с тандемным масс-спектрометрическим детектированием (ВЭЖХ-МС/МС). В
источнике ионов одновременно использовались электрораспыление (ESI) и химическая ионизация
при атмосферном давлении (APCI). Детектирование осуществляли в режиме положительной
ионизации путём мониторинга множественных реакций (MRM). Пробоподготовку плазмы проводили
путём осаждения белков ацетонитрилом. Расчёты при количественном определении проводили
методом внешнего стандарта. Калибровочные кривые носили линейный характер в диапазоне
концентраций 10-5000 нг/мл. Разработанная методика может быть использована для диагностики
острых отравлений гипотензивными лекарственными средствами.
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Литература
1.Rasporjazhenie Pravitel'stva Rossijskoj
Federacii ot 7 dekabrja 2011 g. N 2199-r g.
2.Prikaz Ministerstva zdravoohranenija
Rossijskoj Federacii ot 9 nojabrja 2012 g. N 708n
g. Moskva «Ob utverzhdenii standarta pervichnoj
mediko-sanitarnoj pomoshhi pri pervichnoj
arterial'noj gipertenzii (gipertonicheskoj bolezni)».
3.Prikaz Ministerstva zdravoohranenija
Rossijskoj Federacii ot 24 dekabrja 2012 g. №
1554n «Ob utverzhdenii standarta
specializirovannoj medicinskoj pomoshhi pri
serdechnoj nedostatochnosti».
4.Medicinskaja toksikologija nacional'noe
rukovodstvo. Pod red. E. A. Luzhnikova. M.:
GJeOTAR-Media, 2012. 928 p.
5.Moffat A.C., Osselton M.D., Widdop B.
Clarke's Analysis of Drugs and Poison, London,
Pharmaceutical Press, 2005.
6.K. Czerwiñska E. Wyszomirska T.
Kaniewska. Identification and determination of
selected medicines reducing hypertention by
densitometric and gas chromatographic methods,
Acta Poloniae Phurmaceutica - Drug Research,
2001, Vol. 58, No 5, pp. 331-338.
7.Pujos E, Cren-Olivé C, Paisse O. et al.
Comparison of the analysis of β-blockers by
different techniques, J. Chromatogr. B, 2009, Vol.
877, No 31, pp. 4007-4014.
8.Gonzalez O, Iriarte G, Rico E. et al. LCMS/MS
method for the determination of several
drugs used in combined cardiovascular therapy in
human plasma, J. Chromatogr. B, 2010, Vol. 878,
No 28, pp. 2685-2692.
9.Kristoffersen L., Øiestad E.L., Opdal M.S. et
al. Simultaneous determination of 6 beta-blockers,
3 calcium-channel antagonists, 4 angiotensin-II
antagonists and 1 antiarrhytmic drug in postmortem
whole blood by automated solid phase
extraction and liquid chromatography mass
spectrometry. Method development and
robustness testing by experimental design, J.
Chromatogr. B, 2007, Vol. 850, No 1-2,
pp. 147-160.
10. Gonzalez O., Alonso R.M., Ferreirós N. et
al. Development of an LC-MS/MS method for the
quantitation of 55 compounds prescribed in
combined cardiovascular therapy, J. Chromatogr.
B, 2011, Vol. 879, No 3-4, pp. 243-252.
11. Gu Q., Chen X., Zhong D. et al.
Simultaneous determination of enalapril and
enalaprilat in human plasma by liquid
chromatography–tandem mass spectrometry, J.
Chromatogr. B, 2004, Vol. 813, No 1-2,
pp. 337-342
12. Smyth W.F. Recent studies on the
electrospray ionisation mass spectrometric
behaviour of selected nitrogen-containing drug
molecules and its application to drug analysis
using liquid chromatography–electrospray
ionisation mass spectrometry, J. Chromatogr. B,
2005, Vol. 824, No 1-2, pp. 1-20.
13. Wang D., Jiang K., Yang S. et al.
Determination of nifedipine in human plasma by
ultra performance liquid chromatography–tandem
mass spectrometry and its application in a
pharmacokinetic study, J. Chromatogr. B, 2011,
Vol. 879, No 20, pp. 1827-1832.
14. Li S., Liu G., Jia J. et al. Simultaneous
determination of ten antiarrhythic drugs and a
metabolite in human plasma by liquid
chromatography-tandem mass spectrometry, J.
Мельников и др. / Сорбционные и хроматографические процессы. 2015. Т. 15. Вып. 2
268
Chromatogr. B, 2007, Vol. 847, No 2,
pp. 174-181.
15. Sarkar A.K., Ghosh D., Das A. et al.
Simultaneous determination of metoprolol
succinate and amlodipine besylate in human
plasma by liquid chromatography–tandem mass
spectrometry method and its application in
bioequivalence study, J. Chromatogr. B, 2008,
Vol. 873, No 1, pp. 77-85.
16. Maurer H.H., Tenberken O., Kratzsch C. et
al. Screening for library-assisted identification and
fully validated quantification of 22 beta-blockers
in blood plasma by liquid chromatography–mass
spectrometry with atmospheric pressure chemical
ionization, J. Chromatogr. A, 2004, Vol. 1058,
pp. 169-181.
17. Partani P., Modhave Y., Gurule S. et al.
Simultaneous determination of propranolol and 4-
hydroxy propranolol in human plasma by solid
phase extraction and liquid
chromatography/electrospray tandem mass
spectrometry, Journal of Pharmaceutical and
Biomedical Analysis, 2009, Vol. 50, pp. 966-976.
18. Caban M., Stepnowski P., Kwiatkowski M.
et al. Determination of β-blockers and β-agonists
using gas chromatography and gas
chromatography – mass spectrometry – A
comparative study of the derivatization, J.
Chromatogr. A, 2011, Vol. 1218, No 44,
pp. 8110-8122.
19. Koppel C., Tenczer J. Scope and limitations
of a general unknown screening by gas
chromatography-mass spectrometry in acute
poisoning, J. Am. Soc. Mass Spectrom, 1995, Vol.
6, No 11, pp. 995-1003.
20. Maurer H.H. Systematic toxicological
analysis of drugs and their metabolites by gas
chromatography-mass spectrometry, J.
Chromatogr, 1992, Vol. 580, No 1-2, pp. 3-4.
21. Мельников Е.С., Белова М.В., Раменская
Г.В. Анализ острых отравлений некоторыми
гипотензивными лекарственными веществами
методом газовой хроматографии/масс-
спектрометрии, Масс-спектрометрия, 2014,
Т. 11, № 2, С. 81-88.
22. Maurer H.H. What is the future of (ultra)
high performance liquid chromatography coupled
to low and high resolution mass spectrometry for
toxicological drug screening?, J. Chromatogr. A,
2013, Vol. 1292, pp. 19-24.
23. Руководство по экспертизе
лекарственных средств. Под. ред. проф. А.Н.
Миронова, Том I. М.: Гриф и К, 2013, 328 с.
24. Guidance for Industry: Bioanalytical
method validation. U.S. Department of Health and
Human Services, Food and Drug Administration,
Center for Drug Evolution and Research (CDER).
U.S. Government Printing Office: Washington,
DC. 2001.
25. Guideline on bioanalytical method
validation. European Medicines Agency.
Committee for medicinal products for human use:
London. 2011.
Federacii ot 7 dekabrja 2011 g. N 2199-r g.
2.Prikaz Ministerstva zdravoohranenija
Rossijskoj Federacii ot 9 nojabrja 2012 g. N 708n
g. Moskva «Ob utverzhdenii standarta pervichnoj
mediko-sanitarnoj pomoshhi pri pervichnoj
arterial'noj gipertenzii (gipertonicheskoj bolezni)».
3.Prikaz Ministerstva zdravoohranenija
Rossijskoj Federacii ot 24 dekabrja 2012 g. №
1554n «Ob utverzhdenii standarta
specializirovannoj medicinskoj pomoshhi pri
serdechnoj nedostatochnosti».
4.Medicinskaja toksikologija nacional'noe
rukovodstvo. Pod red. E. A. Luzhnikova. M.:
GJeOTAR-Media, 2012. 928 p.
5.Moffat A.C., Osselton M.D., Widdop B.
Clarke's Analysis of Drugs and Poison, London,
Pharmaceutical Press, 2005.
6.K. Czerwiñska E. Wyszomirska T.
Kaniewska. Identification and determination of
selected medicines reducing hypertention by
densitometric and gas chromatographic methods,
Acta Poloniae Phurmaceutica - Drug Research,
2001, Vol. 58, No 5, pp. 331-338.
7.Pujos E, Cren-Olivé C, Paisse O. et al.
Comparison of the analysis of β-blockers by
different techniques, J. Chromatogr. B, 2009, Vol.
877, No 31, pp. 4007-4014.
8.Gonzalez O, Iriarte G, Rico E. et al. LCMS/MS
method for the determination of several
drugs used in combined cardiovascular therapy in
human plasma, J. Chromatogr. B, 2010, Vol. 878,
No 28, pp. 2685-2692.
9.Kristoffersen L., Øiestad E.L., Opdal M.S. et
al. Simultaneous determination of 6 beta-blockers,
3 calcium-channel antagonists, 4 angiotensin-II
antagonists and 1 antiarrhytmic drug in postmortem
whole blood by automated solid phase
extraction and liquid chromatography mass
spectrometry. Method development and
robustness testing by experimental design, J.
Chromatogr. B, 2007, Vol. 850, No 1-2,
pp. 147-160.
10. Gonzalez O., Alonso R.M., Ferreirós N. et
al. Development of an LC-MS/MS method for the
quantitation of 55 compounds prescribed in
combined cardiovascular therapy, J. Chromatogr.
B, 2011, Vol. 879, No 3-4, pp. 243-252.
11. Gu Q., Chen X., Zhong D. et al.
Simultaneous determination of enalapril and
enalaprilat in human plasma by liquid
chromatography–tandem mass spectrometry, J.
Chromatogr. B, 2004, Vol. 813, No 1-2,
pp. 337-342
12. Smyth W.F. Recent studies on the
electrospray ionisation mass spectrometric
behaviour of selected nitrogen-containing drug
molecules and its application to drug analysis
using liquid chromatography–electrospray
ionisation mass spectrometry, J. Chromatogr. B,
2005, Vol. 824, No 1-2, pp. 1-20.
13. Wang D., Jiang K., Yang S. et al.
Determination of nifedipine in human plasma by
ultra performance liquid chromatography–tandem
mass spectrometry and its application in a
pharmacokinetic study, J. Chromatogr. B, 2011,
Vol. 879, No 20, pp. 1827-1832.
14. Li S., Liu G., Jia J. et al. Simultaneous
determination of ten antiarrhythic drugs and a
metabolite in human plasma by liquid
chromatography-tandem mass spectrometry, J.
Мельников и др. / Сорбционные и хроматографические процессы. 2015. Т. 15. Вып. 2
268
Chromatogr. B, 2007, Vol. 847, No 2,
pp. 174-181.
15. Sarkar A.K., Ghosh D., Das A. et al.
Simultaneous determination of metoprolol
succinate and amlodipine besylate in human
plasma by liquid chromatography–tandem mass
spectrometry method and its application in
bioequivalence study, J. Chromatogr. B, 2008,
Vol. 873, No 1, pp. 77-85.
16. Maurer H.H., Tenberken O., Kratzsch C. et
al. Screening for library-assisted identification and
fully validated quantification of 22 beta-blockers
in blood plasma by liquid chromatography–mass
spectrometry with atmospheric pressure chemical
ionization, J. Chromatogr. A, 2004, Vol. 1058,
pp. 169-181.
17. Partani P., Modhave Y., Gurule S. et al.
Simultaneous determination of propranolol and 4-
hydroxy propranolol in human plasma by solid
phase extraction and liquid
chromatography/electrospray tandem mass
spectrometry, Journal of Pharmaceutical and
Biomedical Analysis, 2009, Vol. 50, pp. 966-976.
18. Caban M., Stepnowski P., Kwiatkowski M.
et al. Determination of β-blockers and β-agonists
using gas chromatography and gas
chromatography – mass spectrometry – A
comparative study of the derivatization, J.
Chromatogr. A, 2011, Vol. 1218, No 44,
pp. 8110-8122.
19. Koppel C., Tenczer J. Scope and limitations
of a general unknown screening by gas
chromatography-mass spectrometry in acute
poisoning, J. Am. Soc. Mass Spectrom, 1995, Vol.
6, No 11, pp. 995-1003.
20. Maurer H.H. Systematic toxicological
analysis of drugs and their metabolites by gas
chromatography-mass spectrometry, J.
Chromatogr, 1992, Vol. 580, No 1-2, pp. 3-4.
21. Мельников Е.С., Белова М.В., Раменская
Г.В. Анализ острых отравлений некоторыми
гипотензивными лекарственными веществами
методом газовой хроматографии/масс-
спектрометрии, Масс-спектрометрия, 2014,
Т. 11, № 2, С. 81-88.
22. Maurer H.H. What is the future of (ultra)
high performance liquid chromatography coupled
to low and high resolution mass spectrometry for
toxicological drug screening?, J. Chromatogr. A,
2013, Vol. 1292, pp. 19-24.
23. Руководство по экспертизе
лекарственных средств. Под. ред. проф. А.Н.
Миронова, Том I. М.: Гриф и К, 2013, 328 с.
24. Guidance for Industry: Bioanalytical
method validation. U.S. Department of Health and
Human Services, Food and Drug Administration,
Center for Drug Evolution and Research (CDER).
U.S. Government Printing Office: Washington,
DC. 2001.
25. Guideline on bioanalytical method
validation. European Medicines Agency.
Committee for medicinal products for human use:
London. 2011.
Опубликован
2018-02-19
Как цитировать
Белова, М. В., Мельников, Е. С., Родина, Т. А., & Раменская, Г. В. (2018). Определение некоторых гипотензивных лекарственных веществ в плазме крови методом высокоэффективной жидкостной хроматографии / тандемной масс-спектрометрии при анализе острых отравлений. Сорбционные и хроматографические процессы, 15(2), 257-268. https://doi.org/10.17308/sorpchrom.2015.15/274
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