Structural and spectral characteristics of composites based on protein conditions with single-walled carbon nanotubes

  • Alexander Yu. Gerasimenko National Research University of Electronic Technology (MIET) 1, Shokin sq., 124498 Moscow, Zelenograd, Russian Federation I.M. Sechenov First Moscow State Medical University (Sechenov University) 2, Bolshaya Pirogovskaya str., bld.4, 119435 Moscow, Russian Federation
  • Dmitry I. Ryabkin National Research University of Electronic Technology (MIET) 1, Shokin sq., 124498 Moscow, Zelenograd, Russian Federation
Keywords: nanocomposites,, laser radiation,, structuring,, framework,, carbon nanotubes,, proteins,, porosity

Abstract

Purpose. The aim of the work is to study nanocomposites created by laser evaporation of waterprotein dispersion of carbon nanotubes. The studies are aimed to determine the features of the interaction between the composite components, as well as to the analysis of the internal and
surface structures of nanocomposites, depending on the type and concentration of the used nanotubes.
Methods and methodology. For the manufacture of nanocomposites single-walled carbon nanotubes of two types were used. Nanotubes of the fi rst type (SWCNTs I) were synthesized by the electric arc method on a Ni/Y catalyst. The average diameter of nanotubes was 1.4–1.8 nm, length ~ 0.3–0.8 microns. Nanotubes of the second type (SWCNTII) were obtained by the method of gas-phase synthesis. The average diameter was ~ 2–3.5 nm, their length was more than 5 microns. To create nanocomposites, a laser method was used to evaporate the waterprotein dispersion of nanotubes. However, the water dispertion of bovine albumin (25 %) and collagen (2 %) was used as a protein matrix. The binding of protein olecules to carbon component was described by Raman spectroscopy. To carry out a comprehensive analysis of the structure and microporosity of nanocomposites, the X-ray microtomography method was used. The study of the specifi c surface and pore volume of the samples was carried out with the use of the method of low-temperature nitrogen porosimetry.
Results. There was a local boiling up of the dispersion of single-walled carbon nanotubes, which was accompanied by the appearance of inhomogeneities in the form of an evolved gas and subsequently produced the production of solid nanocomposites by laser evaporation of the aqueous dispersion of carbon nanotubes with the protein matrix. At the same time, the protein component in nanocomposites undergoes irreversible denaturation. It can be as a biocompatible binding material, which is a source of amino acids for biological tissues during the  mplantation of nanocomposites in the body. While manufacturing of nanocomposites, carbon nanotubes actively absorb radiation, creating an additional thermal heating of the organic components of tissue-engineering structures. As the temperature rises, the number of damages of the weak bonds in the tertiary structure of the protein, what allows the molecule to modify its position in space, increases. It is assumed that the heating from laser radiation and the additional heating from nanotubes accelerate these processes and contribute to the adhesion of the organic
part of the composite to the carbon matrix. The internal structure of the SWCNTI nanocomposites was the most homogeneous. With an increase in concentration from 0.01 to 0.1 %, the average size of micropores increased from 45 to 85 μm and the sample porosity in general from 46
to 58 %. The share of open pores for two types of SWCNTI concentrations was 2 % of the total volume of the composite. SWCNTII-based nanocomposites with both concentrations had a wide range of micropore sizes from 33 to 314 μm. These samples with a concentration of 0.1 % nanotubes had a porosity of up to 73 % with an open pore fraction of 8 %. The presence of mesopores with an average size of up to 46 and 49 nm was found in SWCNTI-based composites, respectively, for concentrations of nanotubes of 0.01 and 0.1 %. An increase in the concentration of 
nanotubes led to a decrease in the specifi c values of the surface and pore volume of the sample.
Conclusions. In this work, nanocomposites were created by laser evaporation of the water-protein dispersion of carbon nanotubes. The features of the interaction between the components of the composite were identifi ed. The analysis of the internal and surface structures of nanocomposites was carried out depending on the type and concentration of the used nanotubes. The structural characteristics of nanoscale composites should positively infl uence on the adhesive characteristics of the lateral processes of cells in various biotissues. The porosity of nanocomposites for biomedical applications is important for triggering the processes of neovascularization and neoinnervation in the repair of defects of biotissues. Thus, the nanocomposites studied in this work can be used as tissue-engineering matrixes to repair bulk defects of biological tissues.

 

REFERENCES

  1. Eletskii A. V. Carbon nanotubes. Usp., 1997, v. 40(9), pp. 899–924. https://doi.org/10.1070/PU1997v040n09ABEH000282
  2. Tuchin A. V., Tyapkina V. A., Bityutskaya L. A., Bormontov E. N. Functionalization of capped ultrashort single-walled carbon nanotube (5, 5). Condensed matter and interphases, 2016, v. 18(4), pp. 568–577. URL: http://www.kcmf.vsu.ru/resources/t_18_4_2016_015.pdf (in Russ.)
  3. Dolgikh I., Tyapkina V. A., Kovaleva T. A., Bityutskaya L. A. 3D Topological changes in enzyme glucoamylase when immobilized on ulrta0short carbon naotubes. Condensed matter and interphases, 2016, v. 18(4), pp. 505–512. URL: http://www.kcmf.vsu.ru/resources/t_18_4_2016_007.pdf (in Russ.)
  4. Kulikova T. V., Tuchin A. V., Testov D. A., Bityutskaya L. A., Bormontov E. N., Averin A. A. Structure and properties of self-organized 2D and 3D antimony/carbon composites. Technical Physics, 2018, v. 63(7), pp. 995–1001. https://doi.org/10.1134/S1063784218070216
  5. Kulikova T. V., Bityutskaya L. A., Tuchin A. V., Lisov E. V., Nesterov S. I., Averin A. A., Agapov B. L. Structural heterogeneities and electronic effects in self-organized core-shell type structures of Sb. Letters on materials, 2017, v. 7(4), pp. 350–354. https://doi.org/10.22226/2410-3535-2017-4-350-354
  6. Gerasimenko A. Yu. Laser structuring of the carbon nanotubes ensemble intended to form biocompatible ordered composite materials. Condensed matter and interphases, 2017, v. 19(4), pp. 489–501. https://doi.org/10.17308/kcmf.2017.19/227
  7. Ma R. Z., Wei B. Q., Xu C. L., Liang J., Wu D. H. The morphology changes of carbon nanotubes under laser irradiation. Carbon, 2000, vol. 38(4), pp. 636–638.  https://doi.org/10.1016/s0008-6223(00)00008-7
  8. Sadeghpour H. R., Brian E. Interaction of laser light and electrons with nanotubes. Physica Scripta, 2004, vol. 110, pp. 262–267. https://doi.org/10.1238/physica. topical.110a00262
  9. Gyorgy E., Perez del Pino A., Roqueta J., Ballesteros B., Cabana L., Tobias G. Effect of laser radiation on multi-wall carbon nanotubes: study of shell structure and immobilization process. of Nanoparticle Research, 2013, v. 15(8), p. 1852. https://doi.org/10.1007/s11051-013-1852-6
  10. Krasheninnikov A. V., Banhart F. Engineering of nanostructured carbon materials with electron or ion beams. Nature Materials, 2007, v. 6(10), pp. 723–733. https://doi.org/10.1038/nmat1996
  11. Ogihara N., Usui Y., Aoki K., Shimizu M., Narita N., Hara K., Nakamura K., Ishigaki N., Takanashi S., Okamoto M., Kato H., Haniu H., Ogiwara N., Nakayama N., Taruta S., Saito N. Biocompatibility and bone tissue compatibility of alumina ceramics reinforced with carbon nanotubes. Nanomedicine, 2012, v. 7(7), pp. 981–993. https://doi.org/10.2217/nnm.12.1
  12. Abarrategi A., Gutiérrez M.C., Moreno-Vicente C., Hortigüela M. J., Ramos V., Lуpez-Lacomba J. L., Ferrer M. L., del Monte F. Multiwall carbon nanotube scaffolds for tissue engineering purposes. Biomaterials, 2008, v. 29(1), pp. 94–102. https://doi.org/10.1016/j.biomaterials.2007.09.021
  13. Newman P., Minett A., Ellis-Behnke R., Zreiqat H. Carbon nanotubes: Their potential and pitfalls for bone tissue regeneration and engineering. Nanomedicine, 2013, v. 9(8), pp. 1139–1158. https://doi.org/10.1016/j.nano.2013.06.001
  14. Sahithi K., Swetha M., Ramasamy K., Selvamurugan N. Polymeric composites containing carbon nanotubes for bone tissue engineering. International journal of biological macromolecules, 2010, v. 46(3). pp. 281–283. https://doi.org/10.1016/j.ijbiomac.2010.01.006
  15. Pan L., Pei, He R., Wan Q., Wang J. Colloids and Surfaces B: Biointerfaces, 2012, vol. 93, pp. 226–234. https://doi.org/10.1016/j.colsurfb.2012.01.011
  16. Mattioli-Belmonte M., Vozzi G, Whulanza Y., Seggiani M., Fantauzzi V., Orsini G., Ahluwalia A. Tuning polycaprolactone–carbon nanotube composites for bone tissue engineering scaffolds. Materials Science and Engineering: C, 2012, v. 32(2), pp. 152–159. https://doi.org/10.1016/j.msec.2011.10.010
  17. Venkatesan J., Qian Z., Ryu B., Kumar N.A., Kim S. Preparation and characterization of carbon nanotube-grafted-chitosan – Natural hydroxyapatite composite for bone tissue engineering. Carbohydrate Polymers, 2011, v. 83(2). pp. 569–577. https://doi.org/10.1016/j.carbpol.2010.08.019
  18. Lin C., Wang Y., Lai Y., Yang W., Jiao F., Zhang H., Shefang Ye., Zhang Q. Incorporation of carboxylation multiwalled carbon nanotubes into biodegradable poly(lactic-co-glycolic acid) for bone tissue engineering. Colloids and Surfaces B: Biointerfaces, 2011, v. 83(2), pp. 367–375.  https://doi.org/10.1016/j.colsurfb.2010.12.011
  19. Gerasimenko A. Yu. , Glukhova O. E., Savostyanov G. V., Podgaetsky V. M. Laser structuring of carbon nanotubes in the albumin matrix for the creation of composite biostructures. Journal of Biomedical Optics, 2017, v. 22(6), pp. 065003-1–065003-8. https://doi.org/10.1117/1.jbo.22.6.065003

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Author Biographies

Alexander Yu. Gerasimenko, National Research University of Electronic Technology (MIET) 1, Shokin sq., 124498 Moscow, Zelenograd, Russian Federation I.M. Sechenov First Moscow State Medical University (Sechenov University) 2, Bolshaya Pirogovskaya str., bld.4, 119435 Moscow, Russian Federation

Gerasimenko Alexander Yu. – Cand. Sci. (Phys.-Math.), Associate Professor, Senior Researcher, National Research University of Electronic Technology MIET, Moscow, Zelenograd, Russian Federation; e-mail: gerasimenko@bms.zone. ORCID iD 0000-0001-6514-2411.

Dmitry I. Ryabkin, National Research University of Electronic Technology (MIET) 1, Shokin sq., 124498 Moscow, Zelenograd, Russian Federation

Ryabkin Dmitry I. – graduate student, National Research University of Electronic Technology MIET, Moscow, Zelenograd, Russian Federation; e-mail: ryabkin@bms.zone. ORCID iD 0000-0002-1327-5690.

Published
2019-06-14
How to Cite
Gerasimenko, A. Y., & Ryabkin, D. I. (2019). Structural and spectral characteristics of composites based on protein conditions with single-walled carbon nanotubes. Kondensirovannye Sredy I Mezhfaznye Granitsy = Condensed Matter and Interphases, 21(2), 191-203. https://doi.org/10.17308/kcmf.2019.21/757
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