Express method for the determination of anions in alcoholic beverages based on the CE-CD combination
Abstract
The control of the chemical composition of alcoholic beverages is an important task, however, the known instrumental methods of analysis do not always allow the identification and quantification of target components, while providing sufficient resolution and sensitivity. In this regard, the development of a highly efficient technique that provides express, highly accurate and reliable identification and quantitative determination of the anionic composition of alcoholic beverages becomes an urgent analytical task, which determined the purpose of this study. The objects of study were model solutions containing: chlorides, nitrates, sulphates, oxalates, formates, fluorides, fumarates, tartrates, malates, citrates, succinates, glycolates, acetates, lactates, phosphates, benzoates and real samples of distilled alcoholic beverages. The study was carried out on Agilent 7100, PrinCE 560 and 750 capillary electrophoresis systems. The analytical signal was recorded using a TraceDec detector by the conductometric method. For the development of the technique, the method of capillary electrophoresis was chosen, the following composition of the buffer solution was selected: 0.2 M HIS,
0.2 MES, 1% Triton X-100, extra pure water, pH 5.5, which provides simultaneous selective determination of 16 anions of organic and inorganic acids in distilled alcoholic drinks. The best conditions and modes of using the capillary electrophoresis system have been worked out, providing maximum separation and sensitivity: voltage +20 kV, hydrodynamic sample injection, conductometric detection, capillary temperature, 24°C, sample injection pressure 30 mbar, sample injection time 25 s, analysis time 10 min. A linear dependence of the analytical signal on the target ion concentration was found in the range of 0.10-20.0 mg/dm3. The limits of the relative error of the measurement technique does not exceed 25%. It has been experimentally confirmed that, under selected conditions, the target ions provide reproducible peak areas and migration times, which allowed carrying out identification based on retention time and quantification using the absolute calibration method. The conducted studies are the basis for the development of a certified method for the simultaneous determination of 16 anions of organic and inorganic acids in 10 minutes in distilled alcoholic beverages.
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References
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