Determination of selective androgen receptor modulators in biologically active supplements by liquid chromatography- mass spectrometry
Abstract
Nowadays a considerable amount of biologically active supplements containing prohibited or not
passed through the clinical trials compounds is being distributed via internet, therefore, there is a need for the
development of procedures to control them. Among such compounds, selective androgen receptor modulators
(SARMs) possessing high anabolic effects and reduced side effects comparing to traditional anabolic steroids
are one of the most popular compounds. Even though these compounds have not passed through the clinical
trials, SARM effects on human body resulted in their popularization, that is why they were added to the Prohibited
List of the World Anti-Doping Agency (WADA) in 2008. Moreover, nowadays compounds related to
other classes and are being distributed under the guise of SARMs and most of them are prohibited by WADA
as well, that is why they should also be controlled.
A procedure for the quantification of selective androgen receptor modulators as well as compounds
distributed under the guise of SARMs via internet has been developed. During the optimization of chromatographic
separation conditions, the use of Luna Omega Polar C18 (2.1×100 mm, 1.6 μm) column and 0.1%
formic acid in water:0.1% formic acid in methanol system using gradient elution was found to be optimum.
Detection of analytes was conducted with the use of a triple quadrupole mass-spectrometer with heated electrospray
ionization operating in negative and positive ion detection modes. To confirm the presence of declared
analytes, high resolution mass-spectrometry, namely a quadrupole-time of flight mass analyzer with
electrospray ionization, was applied. The procedure was used to analyze biologically active supplements
comprising targeted analytes to determine the contents of these analytes. As a result, the contents of analytes
in Ligandrol, Miostop+, Ostarine+, Sarmastol, Radarine drugs correlated with the declared values, while for Andarine, Endorol, Ibutamoren, Reverol+ a deviation from the declared contents was observed (contents
were in the range of 41-83% from the declared values).
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