Development and validation method for quantitative determination taurine and allantoin in chitosan gel by high performance liquid chromatography
Abstract
The subject of the article is the development and validation of a technique for the quantitative determination of taurine and allantoin in the combined presence of chitazan gel, by high-performance liquid chromatography with UV detection. The aim of the work was to develop and validate a technique for the quantitative determination of taurine and allantoin in a chitosan-based gel by HPLC, taking into account sample preparation. The quantitative determination of the gel components was developed taking into account individual sample preparation for each of the active substances and includes the manufacture of two mobile phases for the determination of taurine and allantoin, respectively. The methodology was validated according to the following indicators: specificity, linearity, correctness and precision. The specificity was confirmed by the absence of peaks with retention time of taurine and allantoin in the solvent for sample preparation.
Linearity was confirmed for the analytical region of taurine and allantoin, after constructing a calibration graph and finding the correlation coefficient. Accuracy and precision. To establish the correctness, nine samples were analyzed at three concentration levels for each sample preparation. The accuracy of the technique was determined by the openability index and the value of the response factor. Results of the work: The specificity of the methodology has been established. Linearity was confirmed after establishing the correlation coefficient (r) for taurine 0.9994, for allantoin 0.9995.Correctness and precision were confirmed by the presence of a response factor in the range of 99<R<101%. The data obtained indicate that the technique provides reliable and reproducible results.
Thus, the technique can be used to assess the quantitative content of taurine and allantoin in combined medicines related to elastic-visco-plastic dosage forms similar to a gel with taurine and allantoin based on chitosan. The developed method provides reproducible and accurate results, which allows it to be used to determine the quantitative content of taurine and allantoin at the stages of gel production, and to assess the quality of the gel.
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References
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