A study of histidine sorption by sulfoethylated aminopolymers with subsequent determination by ligand exchange capillary electrophoresis method

  • Vladislav A. Ilin Ural Federal University named after the first President of Russia B. N. Yeltsin, Ekaterinburg
  • Anastasia A. Golota Ural Federal University named after the first President of Russia B. N. Yeltsin, Ekaterinburg
  • Julia S. Petrova 1Ural Federal University named after the first President of Russia B. N. Yeltsin, Ekaterinburg
  • Elena L. Lebedeva 1Ural Federal University named after the first President of Russia B. N. Yeltsin, Ekaterinburg
  • Alexander V. Pestov Ural Federal University named after the first President of Russia B. N. Yeltsin, Ekaterinburg, I.Ya. Postovsky Insititute of Organic Synthesis of the Ural Branch of the Russian Academy of Sciences (IOS UB RAS), Ekaterinburg
  • Ludmila K. Neudachina Ural Federal University named after the first President of Russia B. N. Yeltsin, Ekaterinburg
DOI: https://doi.org/10.17308/sorpchrom.2025.25/12793
Keywords: sorption, amino acids, histidine, aminopolymers, capillary electrophoresis

Abstract

The work is devoted to the development of a technique for the electrophoretic determination of amino acids and the study of the sorption of histidine by materials based on chitosan with a degree of 0.5 sulfoethylation (SEC 0.5), polyethylenimine with a degree of 0.34 sulfoethylation (SEPEI 0.34) and polyamine with degrees of 0.5 sulfoethylation (SEPAS 0.5) and 1.5 (SEPAS 1.5). The development of a technique for the electrophoretic separation and determination of amino acids (alanine, γ-aminobutyric acid, arginine, asparagine, aspartic acid, valine, glycine, histidine, glutamic acid, serine, methionine, lysine, leucine, isoleucine, ornithine, oxyproline, threonine, tyrosine, tryptophan, phenylalanine) was carried out using a capillary monitoring system. electrophoresis "Kapel-105M". As a result of the conducted research, the following conditions for the separation of amino acids were optimized: detection wavelength, temperature, time and method of sample injection, pH and nature of the background electrolyte, concentration of β-cyclodextrin. The developed technique allows for the separation and determination of 12 amino acids when they are present together in solution and the determination of all 20 amino acids under study when they are individually present in solution. The values of the limits of determination and limits of detection of the studied amino acids by ligand exchange capillary electrophoresis under optimized conditions are calculated; concentration ranges of linearity of calibration dependences are determined.

The effect of the pH of an ammonia-acetate buffer solution in the range from 4.0 to 10.0 on the sorption of histidine by sulfoethylated aminopolymers was studied by the limited volume method at an initial amino acid concentration of 1·10-4 mol/dm3 (sorbent weight 0.02 g, solution volume 10.0 cm3). It was found that no quantitative extraction was observed during the sorption of histidine SEC 0.5 and SEPEI 0.34. Extraction of the amino acid SEPAS 1.5 in the sodium form is maximal at pH 4.0 and amounts to 21%. The degree of extraction of the amino acid SEPAS 1.5 in copper form increases with increasing pH and reaches a maximum value of 93% at pH 9.0-10.0. The equilibrium of histidine sorption of SEPAS 0.5 in copper form and SEPAS 1.5 in copper form at pH 9.0 is established within 30 minutes of phase contact.

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Author Biographies

Vladislav A. Ilin, Ural Federal University named after the first President of Russia B. N. Yeltsin, Ekaterinburg

Postgraduate Student, Engineer, Department of Analytical Chemistry and Environmental Chemistry, Institute of Natural Sciences and Mathematics, Ural Federal University, Yekaterinburg, Russia

Anastasia A. Golota, Ural Federal University named after the first President of Russia B. N. Yeltsin, Ekaterinburg

a student at the Department of Analytical Chemistry and Environmental Chemistry of the Institute of Natural Sciences and Mathematics, Ural Federal University, Yekaterinburg, Russia

Julia S. Petrova, 1Ural Federal University named after the first President of Russia B. N. Yeltsin, Ekaterinburg

Head of the Department of Analytical Chemistry and Environmental Chemistry at the Institute of Natural Sciences and Mathematics, PhD, Associate Professor, Ural Federal University, Yekaterinburg, Russia

Elena L. Lebedeva, 1Ural Federal University named after the first President of Russia B. N. Yeltsin, Ekaterinburg

Associate Professor of the Department of Analytical Chemistry and Environmental Chemistry at the Institute of Natural Sciences and Mathematics, Candidate of Chemical Sciences, Ural Federal University, Yekaterinburg, Russia

Alexander V. Pestov, Ural Federal University named after the first President of Russia B. N. Yeltsin, Ekaterinburg, I.Ya. Postovsky Insititute of Organic Synthesis of the Ural Branch of the Russian Academy of Sciences (IOS UB RAS), Ekaterinburg

Associate Professor of the Department of Organic Chemistry and High-Molecular Compounds of the Institute of Natural Sciences and Mathematics, PhD, Associate Professor, Ural Federal University, Yekaterinburg, Russia; Acting Head of the Laboratory of Organic Materials at the I.Ya. Postovsky Institute of Organic Synthesis, Ural Branch of the Russian Academy of Sciences, Yekaterinburg, Russia

Ludmila K. Neudachina, Ural Federal University named after the first President of Russia B. N. Yeltsin, Ekaterinburg

Associate Professor, Department of Analytical Chemistry and Environmental Chemistry, Institute of Natural Sciences and Mathematics, PhD, Associate Professor, Ural Federal University, Yekaterinburg, Russia

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Published
2025-04-04
How to Cite
Ilin, V. A., Golota, A. A., Petrova, J. S., Lebedeva, E. L., Pestov, A. V., & Neudachina, L. K. (2025). A study of histidine sorption by sulfoethylated aminopolymers with subsequent determination by ligand exchange capillary electrophoresis method. Sorbtsionnye I Khromatograficheskie Protsessy, 25(1), 45-55. https://doi.org/10.17308/sorpchrom.2025.25/12793
Issue
Vol 25 No 1 (2025): Sorbtsionnye i Khromatograficheskie Protsessy
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