Cluster method for bilberry anthocyanes analysis using HPLC
Keywords:
reversed-phase HPLC, anthocyanes, cluster analysis, bilberry, juice products.
Abstract
The multicomponent mixtures of anthocyanes from bilberry and blueberry juices were studied by several gradient RP-HPLC modes using octadecyl, hexadecyl, cyanodecyl and phenyl stationary phases. It was shown the separation of all 15 individual glycosides. It was offered a cluster analyses based on peak square normalization for groups of glycosides with the same aglycones. Cluster analysis demonstrates that quantity of each cluster is independent from the type of stationary phase and gradient conditions. It was shown that for bilberry juices the main cluster was based on delfinidine aglycone (34±2%), while for blueberry juices it was based on malvidin aglycone (50±5%). It may be used for the identity determination of juices.
Downloads
Download data is not yet available.
References
1.
Lee J., Rennaker C., Wrolstad R.E., Food Chemistry, 2008, Vol. 110, pp.782-786.
2.
Lee J., Finn C.E., J. Sci. Food Agric., 2007, Vol. 87, pp. 2665-2675.
3.
Lee J., Wrolstad R.E., J. AOAC International, 2005, Vol. 88, No 5, pp. 1269-1278.
4.
Grigoriev A.M., Rudakov O.B., Selemenev V.F., Osobennosti khromatograficheskogo povedeniya antotsianov v usloviyakh gidrofil’noi (obrashchenno-fazovoi) VEZKh. Opredelenie antotsianov v sokosoderzhshchikh napitkakh. In Kromatografiya na blago Rossii. Granitsa, M., 2007, pp. 114-134.
5.
Polina S.А., Efremov А.А., Khimiya rastitelnogo syrya, 2014, Iss. 2, pp. 103-110.
6.
Rukovodstvo po metodam kontrolya kachestva i bezopasnosti biologicheski aktivnykh dobavok k pische, Р 4.1.1672-2004, Minzdrav Rossii, М., 2004.
7.
GOST Р 53773-2010, Produktsiya sokovaya. Metody opredeleniya antotsianov. Standartinform, М., 2010.
8.
Deineka L.А., Shaposhnik Е.I., Gostishchev D.А., Deineka V.I., Sorokopudov V.N., Selemenev V.F., Sorbtsionnye i khromatograficheskie protsessy, 2009, Vol. 9, No 4, pp. 529-536.
9.
European Pharmacopoeia 6th Edition; Supplement 6.2; Fresh Bilberry Fruit Dry Extract Refined and Standardized, pp 3745-3747
10.Kalt W., McDonald J. E., Ricker R. D., Lu X., Canadian Journal of Plant Science, 1999, Vol. 79, No 4, pp.617-623.
Lee J., Rennaker C., Wrolstad R.E., Food Chemistry, 2008, Vol. 110, pp.782-786.
2.
Lee J., Finn C.E., J. Sci. Food Agric., 2007, Vol. 87, pp. 2665-2675.
3.
Lee J., Wrolstad R.E., J. AOAC International, 2005, Vol. 88, No 5, pp. 1269-1278.
4.
Grigoriev A.M., Rudakov O.B., Selemenev V.F., Osobennosti khromatograficheskogo povedeniya antotsianov v usloviyakh gidrofil’noi (obrashchenno-fazovoi) VEZKh. Opredelenie antotsianov v sokosoderzhshchikh napitkakh. In Kromatografiya na blago Rossii. Granitsa, M., 2007, pp. 114-134.
5.
Polina S.А., Efremov А.А., Khimiya rastitelnogo syrya, 2014, Iss. 2, pp. 103-110.
6.
Rukovodstvo po metodam kontrolya kachestva i bezopasnosti biologicheski aktivnykh dobavok k pische, Р 4.1.1672-2004, Minzdrav Rossii, М., 2004.
7.
GOST Р 53773-2010, Produktsiya sokovaya. Metody opredeleniya antotsianov. Standartinform, М., 2010.
8.
Deineka L.А., Shaposhnik Е.I., Gostishchev D.А., Deineka V.I., Sorokopudov V.N., Selemenev V.F., Sorbtsionnye i khromatograficheskie protsessy, 2009, Vol. 9, No 4, pp. 529-536.
9.
European Pharmacopoeia 6th Edition; Supplement 6.2; Fresh Bilberry Fruit Dry Extract Refined and Standardized, pp 3745-3747
10.Kalt W., McDonald J. E., Ricker R. D., Lu X., Canadian Journal of Plant Science, 1999, Vol. 79, No 4, pp.617-623.
Published
2019-11-15
How to Cite
Vasiyarov, G. G., Drob, A. A., Titova, E. V., & Staroverov, S. M. (2019). Cluster method for bilberry anthocyanes analysis using HPLC. Sorbtsionnye I Khromatograficheskie Protsessy, 16(4). Retrieved from https://journals.vsu.ru/sorpchrom/article/view/1374
Section
Статьи
