Use of lithium perchlorate in reversed-phase high performance liquid chromatography of amino compounds
Keywords:
RP-HPLC; ion-pairing agent; lithium perchlorate; amino compounds.
Abstract
The influence of lithium perchlorate as a mobile phase (MP) component on chromatographic
behavior of amino compounds in reversed-phase high performance liquid chromatography (RP-HPLC) was
studied. It was shown that the addition of LiClO4 in MP (pH 2) in both isocratic and gradient elution mode
leads to the increase of retention for protonated bases and to the decrease of their peak asymmetry and peak
widths. It was found that the maximal effect observed at concentrations of LiClO4 in MP ≥ 0.2 M.
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References
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2.Miller J.M. Chromatography. Concepts and contrasts. Wiley-Interscience. A John
Wiley & Sons, Inc., Publication. 2005. 520 p.
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development. Elsevier. 1986. 345 p.
4.Cecchi T. Ion-pair chromatography and related techniques. CRC Press. Taylor &
Francis Group. 2010. 215 p.
5.Chen Y., Mehok A.R., Mant C.T., Hodges R.S. Optimum concentration of
trifluoroacetic acid for reversed-phase liquid chromatography of peptides revisited // J.
Chromatogr. A. 2004. V. 1043. P. 9-18.
6.Juergens U. Alkylamine/phosphoric acid as a universal buffer system for basic and
acidic mobile phases in HPLC // J. of liquid chromatography. 1988. V. 11(9&10). P. 1925-
1940.
7.Schumacher J.C. Perchlorates; their properties, manufacture and uses. Reinhold. New
York. 1960. P. 43.
8.Nevado J.J.B., Peñalvo G.C., Bernardo F.J.G. Simultaneous determination of
suifaquinoxaline, sulfamethazine and pyrimethamine by liquid chromatography // J.
Chromatogr. A. 2000. V. 870. P. 169-177.
9.Дубовик Д.Б., Иванов А.В., Нестеренко П.Н., Тихомирова Т.И. Влияние сильного
электролита в элюенте на удерживание гетерополикислот фосфора и кремния в ион-
парной ВЭЖХ // Вестн. Моск. ун-та. Сер. 2. Химия. 2002. Т. 43. № 5. С. 300-303.
10.Meek J.L. Prediction of peptide retention times in high-pressure liquid chromatography
on the basis of amino acid composition // Proc. Natl. Acad. Sci. USA. 1980. V. 77.
P. 1632-1636.
11.Certa U., Ehrenstein G. Reversed-phase high-performance liquid chromatography of
histones // Anal. Biochem. 1981. V. 118. P. 147-154.
12.Štulik K., Pacákova V., Suchánkova J., Claessens H.A. Srationary phases for peptide
analysis by high performance liquid chromatography: a review // Anal. Chim. Acta. 1997.
V. 352. P. 1-19.
13.Mant C.T., Hodges R.S. Reversed-phase liquid chromatography as a tool in the
determination of the hydrophilicity/hydrophobicity of amino acid side-chains at a ligandreceptor
interface in the presence of different aqueous environments. I. Effect of varying
receptor hydrophobicity // J. Chromatogr. A. 2002. V. 972. P. 45-60.
14.Лобачев А.Л., Колотвин А.А.Идентификация и количественное определение
приоритетных неионогенных и амфотерных поверхностно-активных веществ в
сырье и композициях моющих средств методами ВЭЖХ, тонкослойной
хроматографии и УФ-спектрометрии // Ж. аналит. химии. 2006. Т. 61. №7. С. 678-
685.
15.Molnár I., Horváth C. Separation of amino acids and peptides on non-polar stationary
phases by high-performance liquid chromatography // J. Chromatogr. 1977. V. 142. P. 623-
640.
16.Kanfer I., Skinner M.F., Walker R.B. Analysis of macrolide antibiotics // J.
Chromatogr. A. 1998. V. 812. P. 255-286.
17.LoBrutto R., Jones A., Kazakevich Y.V., McNair H.M. Effect of the eluent pH and
acidic modifiers in high-performance liquid chromatography retention of basic analytes //
J. Chromatogr. A. 2001. V. 913. P. 173-187.
18.LoBrutto R., Jones A., Kazakevich Y.V. Effect of counter-anion on retention in highperformance
liquid chromatography of protonated basic analytes // J. Chromatogr. A.
2001. V. 913. P. 189-196.
19.Jones A., LoBrutto R., Kazakevich Y.V. Effect of the counter-anion type and
concentration on the liquid chromatography retention of β-blockers // J. Chromatogr. A.
2002. V. 964. P. 179-187.
20.Kazakevich Y., LoBrutto R. HPLC for pharmaceutical scientists. A John Wiley &
Sons, Inc. 2007. 1104 p.
21.Dai J., Carr W. Role of ion pairing in anionic additive effects on the separation of
cationic drugs in reversed-phase liquid chromatography // J. Chromatogr. A. 2005.
V. 1072. P. 169-184.
22.Dai J., Mendonsa S.D., Bowser M.T., Lucy C.A., Carr P.W. Effect of anionic additive
type on ion pair formation constants of basic pharmaceuticals // J. Chromatogr. A. 2005.
V. 1069. P. 225-234.
Published
2019-11-18
How to Cite
Azarova, I. N., & Baram, G. I. (2019). Use of lithium perchlorate in reversed-phase high performance liquid chromatography of amino compounds. Sorbtsionnye I Khromatograficheskie Protsessy, 14(1). Retrieved from https://journals.vsu.ru/sorpchrom/article/view/1432
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