Применение CAMAG TLC-MS Interface в двумерной ТСХ: ТСХ – капиллярная ГХ

Aleksey V. Chausov; Victor G. Berezkin; Svetlana V. Khrebtova; Roman S. Borisov; Vladimir G. Zaikin; Tadeousz Н. Dzido

Aleksey V. Chausov graduate student, junior researcher in Topchiev Institute of Petrochemical Synthesis, Moscow
Victor G. Berezkin Professor, doctor of science, chief researcher in Topchiev Institute of Petrochemical Synthesis, Moscow, e-mail: berezkin@ips.ac.ru
Svetlana V. Khrebtova graduate student, junior researcher in Topchiev Institute of Petrochemical Synthesis, Moscow
Roman S. Borisov Ph.D., senior research fellow in Topchiev Institute of Petrochemical Synthesis, Moscow
Vladimir G. Zaikin Professor, doctor of science, head of laboratory in Topchiev Institute of Petrochemical Synthesis, Moscow
Tadeousz Н. Dzido Professor, doctor of science, head of laboratory in Department of Physical Chemistry, Chair of Chemistry, Medical University of Lublin, Poland

Keywords: CAMAG TLC-MS Interface, n-dimensional chromatography, circular TLC, two-dimensional TLC, identification of steroids, separation of chlorophenols.

Abstract

The combined consecutive application of several chromatographic methods (for example, TLC and GC) for
identification of components of the researched mixtures is, undoubtedly, of interest, since it increases its informativity
and reliability. When using TLC as a method of two-dimensional chromatography, the main problem is the transfer of
separated zones of compounds with TLC plate to column chromatography for further separation. To solve this problem,
recently developed the device "CAMAG TLC-MS Interface" (CAMAG) has been applied for the extraction of the zones
from the TLC plate when using the methods of both circular and two-dimensional elution within identifying the
qualitative composition of the individual chromatographic zones on the TLC plates. As objects of the investigation the
mixtures of steroids and chlorophenols were used. The application of CAMAG TLC-MS Interface in n-dimensional
chromatography allowed rapidly and reliably identifying qualitatively separated compounds (what is especially actual
when analyzing the unknown samples). For the first time, it is shown that the device CAMAG TLC-MS Interface can be
used for the different variants of circular (for example, of corner and lateral TLC), two-dimensional TLC and other
variants of TLC

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The application of CAMAG TLC-MS Interface in two-dimensional TLC: TLC – capillary GC

Abstract

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