Comparison of methylene selectivity for reversed-phase and microemulsion the liquid chromatography
Abstract
Advantages of microemulsions as mobile phases in liquid chromatography were shown. The
efficiency in MELC mode was found to be comparable with the efficiency in reversed-phase HPLCmode.
The way to reduce pressure in the chromatographic system in MELC mode was suggested. The
increase of the temperature of the mobile phase leads to the pressure reduction in 1.6 times. The optimal
way to obtain stable microemulsions was worked out. It was shown that the consequent addition of the
components during the microemulsion preparation is necessary to reduce time and temperature of the
microemulsion pretreatment. The selectivity of the microemulsion mobile phases was compared with the
selectivity of the reversed-phase ones. The unique selectivity in the MELC mode was shown. The
retention dependency was found to be linear for all the microemulsion mobile phases.
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References
chromatography. // Anal.Chem. 1983. V.55. № 6. P. 924-928.
2.Ronald R. Eitenmiller W. O., Landen Jr. Vitamin analysis for the health and food
sciences, second edition. 2007. CRC Press. 664p.
3.El-Sherbiny Mohamad D., El-Ashry S. Evaluation of the use of microemulsions as
eluents in high-performance liquid chromatography. // J.Sep.Sci. 2003. V.26. N.6.
P. 503-509.
4.Garti N., Bisperink C. Double emulsions: progress and applications. // Current Opinion
Coll. Inter.Sci. 1998. V.3. N.6. P. 657–667.
5.Malenović A, Ivanović D, Medenica M. Jančić B., Ivanović D. Influence of structural
and interfacial properties of microemulsion eluent on chromatographic separation of
simvastatin and its impurities // J.Chromatogr.A. 2006. V.1131. N.1-2. P.67-73.
6.Malenović A, Medecina M, Ivanović D, Jančić B. Monitoring of Simvastatin
Impurities by HPLC with Microemulsion Eluents // Chromatographia. 2006. V.63.
N.13.(Supplement) P. S95-S100.
7.Marsh A., Clark B., Altria K Oil-in-water microemulsion LC determination of
pharmaceuticals using gradient elution. // Chromatographia. 2005. V.61. N.11. P.539-547.
8.Altria K., Broderick M., Donegan S., Power J. Preliminary study on the use of waterin-
oil microemulsion eluents in HPLC. // Chromatographia. 2005. V.62. N.7.
P. 341-348.
9.Jančić B., Medenica M., Ivanović D. Malenović A., Dimković N. Development of
liquid chromatographic method for fosinoprilat determination in human plasma using
microemulsion as eluent. // J.Chromatogr.A. 2005. V. 1088. №.1-2. P.187-192.
10. Walash M., Belal F., El-Enany N., Abdelal A.N. Microemulsion liquid
chromatographic determination of nicardipine hydrochloride in Pharmaceuticals
preparations and biological fluids. Application to stability studies. // J.Liq.Chromatogr. and
Relat.Technol., 2007. V.30. N.8. P.1015- 1034.
11. Sherbiny D., Mohamad D., El-Ashry S. Evaluation of the use of microemulsions as
eluents in high-performance liquid chromatography. // J.Sep.Sci. 2003. V.26. N.6. P. 503-
509.
12. Дейнека В.И. Хроматографические методы в исследовании биологически
активных веществ растительных материалов. Автореферат дисc. д-ра хим. наук.
Белгород. 2008. 48c.
13. Дейнека В.И. Метиленовая селективность в условиях обращенно-фазовой
хроматографии одного ряда гомологов. // Сорбционные и хроматографические
процессы. Т.7. Вып.2. Белгород. 2007. С.236-243.
