Sorption of cinnamic and hydrocinnamic (coffee and chlorogenic) acids, taxifolin and umbelliferone on active carbon BAU-A
Abstract
Monomeric polyfunctional natural biologically active substances (BAS) of plant origin include cinnamic
and hydroxycoric (coffee, chlorogenic) acids, coumarins (umbelliferon) and Taxifolin, which are contained
in small quantities in almost all medicinal plants (LRS). The aim of this study is to analyze the adsorption
and desorption ability of NPC on active carbon BAU-A.
BAS were adsorbed from solutions of standard samples on BAU-A activated carbon with particle size
2.8-2.0 mm. BAS adsorption was carried out under stationary conditions with laminar mixing (using a Shaker 3.02 mixer) and with turbulent mixing (Vortex type mixer). The time of reaching the sorption equilibrium of BAS and their degree of sorption were experimentally determined. So with laminar mixing, the most complete of sorption of NPC on BAU-A belong to the L type (cinnamic acid and umbelliferone) and the S type (KfK, ChgK, TF) according to the Gibbs classification and confirm the physical mechanism of sorption.
Physicochemical parameters were calculated from adsorption isotherms using the theory of monomolecular adsorption (Freidlinch, Langmuir equations), polymolecular adsorption (BET model) and micropore volumetric theory (Dubinin-Radushkevich equation). The obtained Gibbs energy values confirm that natural phenolic compounds are adsorbed in micropores due to van der Waals forces, and the surface functional groups of AC form hydrogen sorbate-sorbent bonds. The BAS were desorbed under dynamic conditions on a column 180 mm long, 15 mm in diameter, AC layer height 20 mm. As the eluting solutions, there were used polar individual (methanol) and binary (water - ethanol (φ2=0.5), water - acetonitrile (φ2=0.8) solvents. All eluting solvents elute with BAS by 73-83% within 30 min.
Thus, it was found that AC BAU-A is an effective adsorbent in relation to cinnamic and hydrocinnamic
acids, umbelliferone and taxifolin. Methanol or binary mixtures (water - ethanol or water - acetonitrile)
can be used to elute BAS; in this case, almost 83% of BAS can be extracted from the BAU-A sorbent. The
active carbon BAU-A (GOST 6217-74 AO ENPO “Neorganika”, Elektrostal, Moscow Region) is recommended
for the extraction of cinnamic and hydrocinnamic acids, umbelliferone and taxifolin from aqueous
solutions.
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