Определение моногидроксиаренов методом ТСХ

А. П. Асташкина; В. К. Шорманов; О. В. Тарасова; Ю. А. Сухомлинов; О. И. Пугачёва; Л. О. Орехова

doi:10.17308/sorpchrom.2017.17/424

А. П. Асташкина Astashkina Anna P. – candidate of chemical Sciences, associate Professor of physical and analytical chemistry, Institute of natural resources National research Tomsk Polytechnic University, Tomsk, e-mail: apa2004@mail.ru
В. К. Шорманов Shormanov Vladimir K. – Doctor of pharmaceutical science, professor of department of pharmaceutical, toxicological and analytical chemistry, Kursk State Medical University, Kursk, e-mail: R-WLADIMIR@yandex.ru
О. В. Тарасова Tarasova Olga V. – candidate of pharmaceutical Sciences, associate Professor Department of pharmaceutical, Toxicological and analytical chemistry of Kursk state medical University, Kursk
Ю. А. Сухомлинов Suhomlinov Yury A. – candidate of pharmaceutical Sciences, associate Professor of pharmacognosy and botany of Kursk state medical University, Kursk, e-mail: suhoml@yandex.ru
О. И. Пугачёва Pugacheva Oksana I. – postgraduate student of the Department of pharmaceutical, Toxicological and analytical chemistry of Kursk state medical University, Kursk
Л. О. Орехова Orehova Larisa O. – a student of 3 course of pharmaceutical faculty of the Kursk state medical University, Kursk

DOI: https://doi.org/10.17308/sorpchrom.2017.17/424

Keywords: monohydroxylated, normal-phase TLC separation, identification, biological material.

Abstract

We investigated the possibility of using the method of thin-layer chromatography on normal-phase sorbent STH-1A (plate "Sorbfil") for the analysis of some members of the group monohydroxylated, toxic to humans and animals. As objects of study, we have considered the hydroxybenzene, 2,4-dimetilgidrazina, 2,6-dimethylhydroxylamine, 2,4-dimethyl-6-tert-butylhydroxyanisole and 2,6-di-tert-butyl-4-metilgidroksibenzoat. Calculated parameters of chromatographically the analytes in a thin layer of sorbent stkh-1A using a one-, two - and three-component mobile phases of low and medium polarity on the basis of hexane, benzene, chloroform, dioxane and propanol-2. Identified and justified the optimal conditions of separation monohydroxylated and identify them as pure, and after discharge from the biological material by infusion with ethyl acetate. Thus as the movable phase, appropriate use of solvent mixtures hexane-benzene (4:6) (the factor of polarity P=1.24), providing the values of the degree of separation of the most similar chromatographic mobility of the substance in the range of 1.2-3.15 in.

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Identification monohydroxylated in biological material using the method normalizovat TLC

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