Evaluation of charged molecules content of plant polysaccharides by aqueous size exclusion chromatography
Keywords:
size exclusion chromatography, molar mass, heteropolysaccharides, polyelectrolyte expansion, concentration effects
Abstract
Aqueous size-exclusion chromatography (SEC) equipped with refractive index (RI) and multiangle laser light scattering (MALLS) detectors were applied to evaluation of charged molecules content in a series of polysaccharide derivatives with different average molar mass (MM) values. It was shown that a minor amount of residual charged groups in the polysaccharide chains caused a polyelectrolyte expansion effect and neutral fractions of birch xylans, larch arabinogalactans (AG) and galactoglucomannans (GGM) were separated from neutral molecules in various concentrations of the injected samples
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References
1. Barth, H.G., Boyes, B.E., Jackson, Ch. Analytical Chemistry.1998,Vol 70, pp. 251-278.
2. Churms, S.C. J.Chromatogr.Libr, 2002, Vol.66, pp.267-303.
3. Boymirzaev, A.Sorption and chromatographic processes, 2007, Vol.7, No 2, pp. 251-258
4. Porsch, B. Andersson, M. Wittgren, B. Wahlund, K-G., J. Chromatogr. A.2002,Vol.946, pp.69-81
5.Boymirzaev,A.S.Shomurotov,Sh.Turaev,A.S.Chem.plant raw material.2013,No2,pp.51-55.
6. Provder,T. Chromatography of polymers: Characterization by SEC and FFF.1993,337 p.
7. Pasch, H. Trathnigg, B. HPLC of Polymers. Berlin. Springer-Verlag, 1999. 224 p.
8. Dubin P.L. Advances in Chromatography.1992, Vol.31, pp.119-151.
9. Jordan, R.S. J. Liq. Chromatogr. 1980,Vol.3, pp.439-463.
10. Froment, R, Revillon, A. J.Liq.Chromatogr 1987,Vol.10, pp. 1383-1397
11. Eigner, W-D, Abuja, P. Beck, R.H.F. Praznik, W. Carbohydr. Res.1988, Vol. 180, pp. 87-95
12. Song,T,Pranovich,A, Holmbom,B.Bioresource Technology. 2013, Vol.130, pp.198-203
13. Sundberg, A. Holmbom, B. Willför, S. Pranovich, A. Nordic Pulp & Paper Research Journal. 2000, Vol.15, pp. 46-53
14. Kilpeläinen, P. Leppänen, K. Spetz, P. Kitunen, V. Ilvesniemi, H. Pranovich, A. Willför, S. Nordic Pulp and Paper Research Journal. 2012, Vol.27, pp. 680-688
15. Sundberg, A. Sundberg, K. Lillandt, C. Holmbom, B. Nord Pulp Pap.Res.19996, Vol.11, pp. 216-219
16. Eremeeva, T.E., Bykova, T.O. Carbohydrate polymers 1992, Vol.18, pp. 217-219
17. Medvedeva, S.A, Aleksandrova, G.P, Tantsyrev, A.P. Forest Journal. 2002, No 6, pp.108-114
18. Ponder, G.R. Richards, G.N. J. Carbohydr.Chem. 1997, Vol.16, pp.181-193
19. Willför, S. Sjöholm, R. Laine, C. Holmbom, B. Wood Science and Technology, 2002, Vol.36, pp.101-110
20. Stephen, A.M.The Polysaccharides, New.York:Academic Press, 1983,Vol.2, pp.97-103.
2. Churms, S.C. J.Chromatogr.Libr, 2002, Vol.66, pp.267-303.
3. Boymirzaev, A.Sorption and chromatographic processes, 2007, Vol.7, No 2, pp. 251-258
4. Porsch, B. Andersson, M. Wittgren, B. Wahlund, K-G., J. Chromatogr. A.2002,Vol.946, pp.69-81
5.Boymirzaev,A.S.Shomurotov,Sh.Turaev,A.S.Chem.plant raw material.2013,No2,pp.51-55.
6. Provder,T. Chromatography of polymers: Characterization by SEC and FFF.1993,337 p.
7. Pasch, H. Trathnigg, B. HPLC of Polymers. Berlin. Springer-Verlag, 1999. 224 p.
8. Dubin P.L. Advances in Chromatography.1992, Vol.31, pp.119-151.
9. Jordan, R.S. J. Liq. Chromatogr. 1980,Vol.3, pp.439-463.
10. Froment, R, Revillon, A. J.Liq.Chromatogr 1987,Vol.10, pp. 1383-1397
11. Eigner, W-D, Abuja, P. Beck, R.H.F. Praznik, W. Carbohydr. Res.1988, Vol. 180, pp. 87-95
12. Song,T,Pranovich,A, Holmbom,B.Bioresource Technology. 2013, Vol.130, pp.198-203
13. Sundberg, A. Holmbom, B. Willför, S. Pranovich, A. Nordic Pulp & Paper Research Journal. 2000, Vol.15, pp. 46-53
14. Kilpeläinen, P. Leppänen, K. Spetz, P. Kitunen, V. Ilvesniemi, H. Pranovich, A. Willför, S. Nordic Pulp and Paper Research Journal. 2012, Vol.27, pp. 680-688
15. Sundberg, A. Sundberg, K. Lillandt, C. Holmbom, B. Nord Pulp Pap.Res.19996, Vol.11, pp. 216-219
16. Eremeeva, T.E., Bykova, T.O. Carbohydrate polymers 1992, Vol.18, pp. 217-219
17. Medvedeva, S.A, Aleksandrova, G.P, Tantsyrev, A.P. Forest Journal. 2002, No 6, pp.108-114
18. Ponder, G.R. Richards, G.N. J. Carbohydr.Chem. 1997, Vol.16, pp.181-193
19. Willför, S. Sjöholm, R. Laine, C. Holmbom, B. Wood Science and Technology, 2002, Vol.36, pp.101-110
20. Stephen, A.M.The Polysaccharides, New.York:Academic Press, 1983,Vol.2, pp.97-103.
Published
2018-02-22
How to Cite
Willför, S., Boymirzaev, A. S., & Pranovich, A. (2018). Evaluation of charged molecules content of plant polysaccharides by aqueous size exclusion chromatography. Sorbtsionnye I Khromatograficheskie Protsessy, 17(4), 689-694. https://doi.org/10.17308/sorpchrom.2017.17/429
Section
Статьи
