The study of amino acid composition of herb Scabiosa ochroleuca L. by HPLC

  • Irina L. Drozdova the doctor of pharmaceutical Sciences, the Dean of the pharmaceutical and biotechnological faculties, Professor of the Department of pharmacognosy and botany of the Kursk state medical University, Kursk, E-mail: irina-drozdova@yandex.ru
  • Eugenia I. Minakova student of pharmaceutical faculty of the Kursk state medical University, Kursk
Keywords: Scabiosa ochroleuca L., amino acid composition, high performance liquid chromatography (HPLC).

Abstract

Purpose of work was to analyze the amino acid composition of the herb Scabiosa ochroleuca L. family Dipsacaceae by high performance liquid chromatography (HPLC). The object of the study was air-dried chopped herb Scabiosa ochroleuca L., harvested in the flowering phase. The presence of amino acids set qualitative reactions. To study the content of amino acids was applied the method of high performance liquid chromatography (HPLC). The contents of amino acids were determined using amino acid analyzer (Amino Acid Analyzer AAA 400).

Qualitative reactions proved the presence of amino acids. By HPLC revealed the existence of 15 amino acids (12 aliphatic; 1 heterocyclic and 2 aromatic amino acids, which is consistent with the literature data on the content of amino acids in plants). 7 acids are essential (threonine, valine, methionine, isoleucine, leucine, phenylalanine, lysine). The content of amino acids makes up 4,94%, including essential amino acids of 1,66%. At present the predominant concentration of glutamic acid (0,63%). The amino acid composition of the investigated plants was studied for the first time. The results indicate the prospects of using plants as a source of amino acids. In combination with other groups of biologically active substances (phenolic compounds, polysaccharides, macro - and microelements and other) it emphasizes the therapeutic importance of herbs Scabiosa ochroleuca L. and enables the development of new affordable domestic medicines combined action on the basis of promising species of medicinal plants.

Downloads

Download data is not yet available.

References

1. Gosudarstvennyi reestr lekarstvennykh sredstv. Rezhim dostupa: http://www.grls.rosminzdrav.ru (data obrashcheniya 17.05.2017)
2. Kkhaled Abu Zakher, Zhuravlev N.S. Aminokislotnyi sostav nekotorykh vidov rastenii roda Rumex L. // Elektronnyi zhurnal «Provizor». №21. 2001. http://www.provisor.com.ua/
3. Dikorastushchie poleznye rasteniya Rossii. / Otv. red. A.L. Budantsev, E.E. Lesiovskaya, SPb., Izd-vo SPKhFA, 2001, 663 p.
4. Maevskii P.F. Flora srednei polosy Evropeiskoi chasti Rossii, M., Tovarishchestvo nauchnykh izdanii KMK, 2006, 600 p.
5. Prudnikov N.A., Poluyanov A.V., Sosudistye rasteniya Kurskoi oblasti, Kursk, KGU, 2005, 80 p.
6. Rastitel'nye resursy Rossii: Dikorastushchie tsvetkovye rasteniya, ikh komponentnyi sostav i biologicheksaya akivnost'. T.4. Semeistva Caprifoliaceae-Lobeliaceae / Otv. red. A.L. Budantsev, SPb.; M., Tovarishchestvo nauchnykh izdanii KMK, 2011, 630 p.
7. Krupennikova V.G. Farmakognosticheskoe issledovanie Scabiosa comosa i Scabiosa ochroleuca L., proizrastayushchikh v Vostochnoi Sibiri. Avtoref. dis. kand. farm. nauk, Ulan-Ude, 2007, 15 p.
8. Sergunova E.V., Sorokina A.A.,, Farmatsiya, 2012, No 5, pp. 11-13.
9. Mironenko N.V., Brezhneva T.A., Mikhina I.A., Selemenev V.F., Zavodskaya laboratoriya. Diagnostika materialov, 2009, Vol. 75, No 5, pp. 19-23.
10. Trineeva O.V., Sinkevich A.V., Slivkin A.I., Khimiya rastitel'nogo syr'ya, 2015, No 2, pp. 141-148.
11. Kiseleva T.L., Lyui G., Chauzova A.V., Traditsionnaya meditsina, 2014, No 1 (36), pp. 49-52.
12. Drozdova I.L., Denisova N.N., Traditsionnaya meditsina, 2012, No 4 (31), pp. 49-51.
13. Drozdova I.L., Lupilina T.I., Vestnik Voronezhskogo gosudarstvennogo universiteta. Seriya: Khimiya. Biologiya. Farmatsiya, 2015, No 1, pp. 125-128.
14. Trineeva O.V., Sinkevich A.V., Slivkin A.I., Safonova E.F., Sorbtsionnye i khromatograficheskie protsessy, 2014, Vol. 14, No 3, pp. 530-536.
15. Trineeva O.V., Slivkin A.I., Dmitrieva A.V., Razrabotka i registratsiya lekarstvennykh sredstv, 2014, No 9, pp. 136-142.
16. Shilova I.V., Kuvacheva N.V., Samylina I.A., Farmatsiya, 2016, Vol. 65, No 4, pp. 35-38.
Published
2018-02-27
How to Cite
Drozdova, I. L., & Minakova, E. I. (2018). The study of amino acid composition of herb Scabiosa ochroleuca L. by HPLC. Sorbtsionnye I Khromatograficheskie Protsessy, 18(1), 52-57. https://doi.org/10.17308/sorpchrom.2018.18/464