Investigation of competitive sorption of influenza virus and bacteria on polymeric materials
Abstract
The purpose of this work was to establish the possibility of using electrically conductive polymers to remove different types of micropathogens that are simultaneously present in aqueous media
As sorbents for micropathogens, polypyrrole (PPy) and polyaniline (PANI) composites without and containing silver ions, differing in shape, chemical structure and properties were used. It was sorbed : the virus - influenza B, line B/Victoria/2/87, similar to the epidemic strains that circulated during the period 2016-2017 in Russia and the world and representatives of bacteria Gram (+)) bacteria - Staphylococcus aureus and Gram-negative (Gram (-)) bacteria: Shigella flexneri 2a. Shigella flexneri 2a. Staphylococcus aureus are conditionally pathogenic microorganisms that are present as in healthy people, as in the many people for several months, and also cause numerous life-threatening zooanthroponotic infections. Shigella flexneri 2a is the causative agent of an acute intestinal infection, characterized by the development of secondary immunodeficiencies, protracted and chronic forms of infectious diseases, and has a predominantly waterway pathway. Biological models had the differed morphology and structure of proteins.
The experiment was conducted in several stages. At the first stage, sorption of individual micro pathogens was carried out for all types of polymer sorbents: the influenza virus and each type of bacteria. To do this, the micropathogen was contacted with the sorbent in the aqueous phase, precipitation was performed with low-speed centrifugation of the complex formed, the activity of the virus in the supernatant and in the complex was analyzed.
To determine the intensity of sorption in solution, a comparison the number of pathogen was made before and after sorption (in the case of influenza viruses of hemagglutinating titers) and (in the case of bacteria) the number of viable bacteria after compared with the control of the corresponding dilutions after seeding of the test samples with 2% nutrient agar.
In the main experiment, samples containing virus + sorbent complexes were placed in an aqueous solution into which one type of bacteria was added. After contact and centrifugation the efficiency of bacterial sorption for each species was evaluated both in the number of viable bacteria in the supernatant and as in the virus + sorbent + bacteria complex, When comparing the adsorption properties of polymers with the sorbet virus, it was shown that the greatest bacteria sorption activity is decreased in the following sequence of PANI> PPy-Ag> PANI-Ag> PPy sorbents for the bacteria Shigella flexneri 2a and PANI> PANI-Ag> PPy-Ag> PPy for bacteria Staphylococcus aureus. High sorption characteristics allow using these materials for two aims - anti viral and anti bacterial filters simultaneously .
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References
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