Prospects for using the TLC method for identifying and assessing the quality of plant raw ivy
Abstract
Various ivy types are perspective for medicinal use, they contain up to 10% of triterpenic saponins n leaves and have a wide spectrum of biological activity. In this regard, the studies of the standardization of ivy plant raw materials, variability of its chemical composition, climatic features, and soil composition of the
plant habitat are relevant. The aim of this study was determination of TLC method prospects for identifying and assessing the quality of ivy common raw materials, as well as evaluating the variability of chromatographic profiles depending on the plant habitat. As object of the study were used dried, chopped ivy common leaves collected in the summer period from fruitless plant shoots growing in different geographical areas: Russia (Caucasus, Crimea), Iraq, Italy. Optimal TLC conditions testing was carried out using the sum of saponins of ivy leaves extract – «the Hederix+» phytocomplex, produced by «Vitaukt-prom» LLC (Russia), as a standard.
The optimal conditions (including eluent, detection reagent, plate type, method of chromatography, sample volume) for chromatographing the amount of ivy saponins in a thin layer of sorbent were studied and selected. The optimal conditions for the process of chromatography are the following: highly efficient Sorbfill PTSH-P-V plates, sample volume 10 μl, eluent chloroform-methanol-water 100: 25: 3, detection reagent -0.2% para-oxybenzaldehyde alcohol solution in 1 M sulfuric acid.Сhromatographic profiles of ivy leaves extracts were obtained in the aforesaid conditions. The obtained chromatograms are quite informative and can be used as «fingerprints» for ivy raw materials identification, since they contain from 9 (Italy, Crimea) to 12 (Iraq) saponin zones. More than 10 zones of related compounds are also detected on the chromatograms viewed in visible and UV light. The «fingerprints» differences indicate the variability of the chromatographic profiles of methanol ivy leaves extracts grown in different geographical areas. The optimal conditions for the TLC analysis of saponin-containing ivy common leaves extracts were determined.
Chromatographic profiles of extracts were obtained, the possibility of using them as «fingerprints» to identify ivy plant raw materials was shown. The variability of chromatographic profiles of ivy leaves extracts and its dependence on the plant habitat were identified. The obtained data can be recommended for use in the regulatory documentation development.
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