Catalytic properties of glutathione peroxidase under toxic liver damage and the administration of 6-hydroxy-2,2,4-trimethyl-1,2-dihydroquinoline in rats

  • Darya A. Brazhnikova the student, Voronezh State University, Voronezh, е-mail: dasha. brazhnikowa@yandex.ru (correspondence writer)
  • Evgenii D. Kryl'skii Ph.D. (biology), assistant, department of medical biochemistry and microbiology, Voronezh State University, Voronezh, е-mail: evgenij.krylsky@yandex.ru
  • Larisa V. Matasova Ph.D. (biology), associate prof., department of medical biochemistry and microbiology, Voronezh State University, Voronezh, е-mail: larissamatasova@bio.vsu.ru
  • Кonstantin K. Shulgin Ph.D. (biology), associate prof., department of medical biochemistry and microbiology, Voronezh State University, Voronezh, е-mail: kkshulgin@mail.ru
  • Aleksei N. Verevkin Ph.D. (biology), assistant, department of medical biochemistry and microbiology, Voronezh State University, Voronezh, е- mail: wer.all@mail.ru
  • Sergei S. Popov grand Ph.D (medicine, MD), prof., department of hospital therapy and endocrinology, Voronezh State Medical University named after N. N. Burdenko, Voronezh, е-mail: popovendo@ mail.ru
  • Tatyana N. Popova prof., grand Ph.D (biology), department of medical biochemistry and microbiology, Voronezh State University, Voronezh, е-mail: biomed-popova@yandex.ru
DOI: https://doi.org/10.17308/sorpchrom.2019.19/787
Keywords: glutathione peroxidase, toxic liver damage, 6-hydroxy-2,2,4-trimethyl-1,2- dihydroquinoline, oxidative stress.

Abstract

The study is devoted to clarifying the regulatory and catalytic properties of glutathione peroxidase from liver of rats with toxic liver damage and 6-hydroxy-2,2,4-trimethyl-1,2-dihydroquinoline exposure. The pathology was induced by intragastric administration of carbon tetrachloride at a dose of 0.064 cm3 per 100 g of body weight. The test compound was orally administered at a dose of 50 mg/kg., Animals were divided into 3 groupsduring the experiment: control rats, animals with toxic liver damage, and rats receiving 6-hydroxy-2,2,4-trimethyl-1,2-dihydroquinoline against the background of the pathology. For the purification of glutathione peroxidase from rat liver, chromatographic separation methods with Sephadex G-25 and DEAE-cellulose were used. Glutathione peroxidase activity was measured by a conjugate reaction with glutathione reductase using the Warburg test. The protein content was assessed using the BCA protein assay kit. The results showed that in rats treated with 6-hydroxy-2,2,4-trimethyl-1,2-dihydroquinoline against the background of the pathology induction, the pH optimum and glutathione peroxidase affinity for glutathione were changed in the controldirection. The obtained data may indicate a change in the conformational properties of the enzyme under conditions of a decrease the free radical oxidation intensity under the test compoundaction.
In addition, the introduction of 6-hydroxy-2,2,4-trimethyl-1,2-dihydroquinoline contributed to the normalization of the glutathione peroxidase regulatory properties, to respect toisocitrate, glucose-6-phosphate and Fe2+. Thus, the results of the work indicate the ability of 6-hydroxy-2,2,4-trimethyl-1,2-dihydroquinoline to have a corrective effect on the glutathione peroxidase catalytic and regulatory properties due, apparently, to the antioxidant properties manifestation by this compound.

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Published
2019-07-23
How to Cite
Brazhnikova, D. A., Kryl’skii, E. D., Matasova, L. V., ShulginК. K., Verevkin, A. N., Popov, S. S., & Popova, T. N. (2019). Catalytic properties of glutathione peroxidase under toxic liver damage and the administration of 6-hydroxy-2,2,4-trimethyl-1,2-dihydroquinoline in rats. Sorbtsionnye I Khromatograficheskie Protsessy, 19(4), 481-488. https://doi.org/10.17308/sorpchrom.2019.19/787
Issue
Vol 19 No 4 (2019): Sorbtsionnye i Khromatograficheskie Protsessy
Section
Статьи