Biocatalysts based on papain associates with chitosan nanoparticles
Abstract
The research purpose was to develop and study biocatalysts based on papain associates with chitosan nanoparticles. We obtained medium and high molecular weight chitosan nanoparticles, both with and without ascorbic acid .
When the papainna-noparticles complexes with ascorbic acid were formed, the catalytic activity of the enzyme increased by 3 % for medium molecular weight chitosan and by 16 % for high molecular weight chitosan. After 168 hours of incubation in 0.05 M of Tris-HCl buffer (pH 7.5) at 37 °C, the free enzyme retained 15 % of its catalytic activity, whereas its associates with chitosan nanoparticles exhibited ~ 30 %. The papain complex with chitosan nanoparticles and ascorbic acid exhibited 40 % of the enzyme catalytic activity.
We simulated the bonds and interactions within the chitosan-ascorbic acid-papain complex. The proposed biocatalysts have high prospects for effective use in cosmetology, biomedicine, and pharmacy.
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