PHYSICO-CHEMICAL VERIFICATION OF THE APPLICATION OF THE CYTOSTATIC IMMOBILAZED FORM IN OVARIAN CANCER THERAPY
Abstract
The aim of the research is to study the interaction between "Linteks-Mesogel" and paklitaksel, antitumor pharmaceutical substances. The object of the research is "Linteks-Mesogel", a concentrated water solution of sodium salt of carboxymethyl cellulose (Na-CMC). It can be used as an inert polymer carrier for some pharmaceutical substances and has an anti-spasmodic effect. FTIR and UV-spectroscopy methods were used to investigate the interaction. The UV-spectroscopy showed that light absorbance maxima for paklitaksel and its mixture with "Linteks-Mesogel" water solution are identical. The FTIR data confirm absence of covalent bonding between the molecules of sodium salt of carboxymethyl cellulose and the molecules of paklitaksel. A shift to 1642 cm-1 can only be observed in case of the absorption band at 1636 cm-1, which is attributed to stretching vibrations of the carbonyl groups of sodium salt of carboxymethyl cellulose in associated water solutions. It proves that there is destabilization of the association of Na-CMC macromolecules in aqua solutions with paklitaksel molecules which causes the formation of self-organizing systems. The transmission electron microscopy (TEM) showed that the associates of Na-CMC with paklitaksel have a complex composition. The associates consisting of a number of Na-CMC macromolecules and paklitaksel molecules form self-organizing systems with a strictly defined architecture. To sum up, the obtained data allow us to conclude that "Linteks-Mesogel" can be used as polymer carrier for paklitaksel in the ovarian cancer therapy providing a prolonged action and a decreased toxicity of paklitaksel.
ACKNOWLEDGEMENTS
The FTIR and TEM data were obtained with equipment of the Centre for Collective Use of Scientific Equipment of Voronezh State University.
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