Применение магнитного сорбента на основе гумата в сочетании с ГХ-МС для определения фенольных ксеноэстрогенов в донных отложениях
Аннотация
Сорбент с магнитными свойствами, функционализированный гуматами, в сочетании с газовой хроматографией – масс-спектрометрией (ГХ-МС) предложен для определения фенольных ксеноэстрогенов (ED) в донных отложениях. В качестве объектов исследования выбраны ED – октилфенол (ОР), нонилфенол (NP), бисфенол А (BPA). Вместе с ED изучено распределение эстрогена естественного происхождения – 17β-эстрадиола (Е2). Предварительно ED извлекали из донных отложений гексаном при кратковременном озвучивании ультразвуком. Степень извлечения компонентов составила 75-89%. Сорбционное концентрирование проводили в динамических условиях: сорбент массой 0.5 г помещали в колонку из боросиликатного стекла, с двух сторон которой размещали магниты для иммобилизации сорбента. Гексановый экстракт пропускали через колонку, заполненную магнитным сорбентом на основе гуматов. Затем аналиты десорбировали 1 см3 метанола и проводили их дериватизацию проводили смесью N,O-бис(триметилсилил) -трифторацетамида и триметилхлорсилана.
Аналитические характеристики способа определения устанавливали на примере модельных образцов донных отложений, отобранных в фоновой местности с минимальным антропогенным воздействием. Предел определения ED составил 30-60 нг/кг (сухой массы). В реальных объектах чувствительность способа снижается в 3-4 раза за счет матричных эффектов, обусловленных присутствием нефтепродуктов в водах. Проведен мониторинг содержания ED в донных отложениях в месте сброса сточных вод в р. Дон у г. Воронежа, а также на Черноморском побережье Кавказа (район г. Туапсе и пос. Ольгинка). Максимальные концентрации ОР, NP, BPA и Е2 в донных отложениях установлены в районе порта г. Туапсе, где они составили 5.7, 8.1, 6.2 и 0.9 мкг/кг соответственно.
Скачивания
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