Comparison of traditional and continuous HPLC buserelin chromatographic purification methods
Keywords:
preparative HPLC, peptide purification, buserelin, continuous chromatography, MCSGP.
Abstract
Project scope: examination of different reversed-phase HPLC chromatographic purification methods of anticancer synthetic buserelin peptide in traditional isocratic or gradient batch conditionals and in multicolumn continuous chromatography (MCSGP). Process optimization was done in isopropanol-water solutions with citric acid additives.
It has been demonstrated that transition from the batch to continuous process raises the productivity by 1.5-3 times and increases the product yield from 56-61% to 85% with saving the demands for the product purity (more than 97%).
Downloads
Download data is not yet available.
References
1.Terent'ev A.A., Lychkova A.E., Kazimirskii A.N., Salmasi Zh.M., Eksperimental'naya i klinicheskaya gastroenterologiya, 2014, Vol. 112 (12), pp. 61-63.
2.Krivitskaya V.Z. et al., Klinicheskaya laboratornaya diagnostika, 2012, No 5, pp. 42-45.
3.Khasanova L.Kh. et al., Vestnik RUDN. ser. Meditsina. Akusherstvo i ginekologiya, 2002, No 1, pp. 225-227.
4.Rassmusen P.Kh., Rassmusen I.Kh., Patent RU № 2303040, Ochistka peptidov.
5.Mikhailova A.A. et al., Patent RU 2283663, Immunomodulyator s protivoopukholevoi aktivnost'yu i lekarstvennoe sredstvo na ego osnove.
6.Ludemann-Hombourger O., Specialty Chemicals Magazine, 2013, pp.30.
7.Aumann L., Morbidelli M., Biotech. & Bioeng., 2008, Vol. 99(3), pp. 728-733.
8.Aumann L., Stroehlein G., Schenkel B., Morbidelli M, Biopharm. Int., 2008, pp. 22(1).
9.Vickery B.H., Endocr. Rev., 1986, Vol. 7, pp. 115-124.
10.
Hoffken K., Recent Results Cancer Res., 1992, Vol. 124. pp. 91-104.
11.
Santen R.J., Manni A. Harvey H., Breast Cancer Res. Treat., 1986, Vol. 7, pp. 129- 145.
12.
Hazun E., Conn P.M., Endocr. Rev., 1988, Vol. 9, pp. 379-386.
13.
Imre Mezo et al., J. Med. Chem., 1997, Vol. 40, pp. 3353-3358.
14.
Karasev V.S., Predel'skii A.D., Staroverov S.M. Patent RU № 2332248 S1, 2007. Sposob khromatograficheskoi ochistki buserelina.
15.
Wuttke W. et al., Acta neurobiologiae experimentalis, 1996, Vol. 56 (3), pp. 707-713.
16.
Mutschler E., Schäfer-Korting M., Wissenschaftliche Verlagsgesellschaft, 2001, Vol. 8, pp. 372–373.
2.Krivitskaya V.Z. et al., Klinicheskaya laboratornaya diagnostika, 2012, No 5, pp. 42-45.
3.Khasanova L.Kh. et al., Vestnik RUDN. ser. Meditsina. Akusherstvo i ginekologiya, 2002, No 1, pp. 225-227.
4.Rassmusen P.Kh., Rassmusen I.Kh., Patent RU № 2303040, Ochistka peptidov.
5.Mikhailova A.A. et al., Patent RU 2283663, Immunomodulyator s protivoopukholevoi aktivnost'yu i lekarstvennoe sredstvo na ego osnove.
6.Ludemann-Hombourger O., Specialty Chemicals Magazine, 2013, pp.30.
7.Aumann L., Morbidelli M., Biotech. & Bioeng., 2008, Vol. 99(3), pp. 728-733.
8.Aumann L., Stroehlein G., Schenkel B., Morbidelli M, Biopharm. Int., 2008, pp. 22(1).
9.Vickery B.H., Endocr. Rev., 1986, Vol. 7, pp. 115-124.
10.
Hoffken K., Recent Results Cancer Res., 1992, Vol. 124. pp. 91-104.
11.
Santen R.J., Manni A. Harvey H., Breast Cancer Res. Treat., 1986, Vol. 7, pp. 129- 145.
12.
Hazun E., Conn P.M., Endocr. Rev., 1988, Vol. 9, pp. 379-386.
13.
Imre Mezo et al., J. Med. Chem., 1997, Vol. 40, pp. 3353-3358.
14.
Karasev V.S., Predel'skii A.D., Staroverov S.M. Patent RU № 2332248 S1, 2007. Sposob khromatograficheskoi ochistki buserelina.
15.
Wuttke W. et al., Acta neurobiologiae experimentalis, 1996, Vol. 56 (3), pp. 707-713.
16.
Mutschler E., Schäfer-Korting M., Wissenschaftliche Verlagsgesellschaft, 2001, Vol. 8, pp. 372–373.
Published
2019-11-15
How to Cite
Kiryanov, A. Y., Salionov, D. S., Karasev, V. S., & Staroverov, S. M. (2019). Comparison of traditional and continuous HPLC buserelin chromatographic purification methods. Sorbtsionnye I Khromatograficheskie Protsessy, 16(4). Retrieved from https://journals.vsu.ru/sorpchrom/article/view/1373
Section
Статьи
