Extraction technologies for clinical HPLC analysis of serum progesterone, 17α-hydroxyprogesterone and synthetic progestin’s
Keywords:
17a-hydroxyprogesterone, progesterone, liquid-liquid extraction (LLE), solid-phase extraction (SPE), hyper cross-linked polystyrene, HPLC.
Abstract
The methods of liquid-liquid extraction (LLE) and solid-phase extraction (SPE) from human serum
for 17a-hydroxyprogesterone and progesterone, as well as, synthetic progestin's are developed. The analysis
carried out by means of conventional reversed-phase HPLC with UV detection. LLE made by mean of
pentane – dichloromethane/chloroform, SPE on cartridges packed 30 mg hyper cross-linked polystyrene.
Simplicity, reproducibility both sufficient selectivity and sensitivity of a method, allow to use it in a clinical
practice for an estimation of the hormonal status at women, including receiving synthetic steroid drugs.
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References
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полистирола как сорбента для твердофазной экстракции при анализе лекарств в
биологических объектах методом высокоэффективной тонкослойной хроматографии
(ВЭТСХ) // Сорбционные и хроматографические процессы. 2010. Т. 10. вып. 1.
С. 5-14.
3. Клиническая эндокринология (руководство), под ред. Н.Т. Старковой. Питер.
СПб. 2002. 576 с.
4. Сметник В.П., Тумилович Л.Г. Неоперативная гинекология. Сотис. СПб. 1994.
224 с.
5. Энциклопедия клинических лабораторных тестов. М.: Лабинформ, 1997. 960 с.
6. Alvarez S.B, Capote F.P., Jimenez J.R., Lique de Castro M.D. Automated solidphase
extraction for concentration and clean-up of female steroid hormones prior to liquid
chromatography-electrospray ionization-tandem mass spectrometry: an approach to
lipidomics // J. Chromatogr. A. 2008. V. 1207(1-2). P. 46-54.
7. Etter M.L, Eichhorst J., Lehotay D.C. Clinical determination of 17-
hydroxyprogesterone in serum by LC-MS/MS: comparison to Coat-A-Count RIA method
// J. Chromatogr. B. Analyt Technol Biomed Life Sci. 2006. V. 840(1). P. 69-74.
8. Fanelli F, Belluomo I, Di Lallo V.D. et al. Serum steroid profiling by isotopic
dilution-liquid chromatography-mass spectrometry: comparison with current
immunoassays and reference intervals in healthy adults // Steroids. 2011. V. 76(3). P. 244-
253.
9. Kabra P.M. Clinical analysis of individual steroids by column liquid chromatography
(review) // J. Chromatogr.B. 1988. V. 429. P.155-176.
10.Kartsova L.A., Bessonova E.A. Determination of Steroids in Biological Samples by
Micellar Electrokinetic Chromatography // J. of Analytical Chemistry. 2007. V. 62. N. 1.
P. 68-75.
11.Kuhn R.W., Deyman M.E. Simultaneous quantification of natural glucocorticoids
and progestins in serum // J. Chromatogr.-B. 1987. V. 421. P. 123-129.
12.Naumann J.M., Zöllner A., Drăgan C.A. et al. Biotechnological production of 20-
alpha-dihydrodydrogesterone at pilot scale // Appl Biochem Biotechnol. 2011. V. 165(1).
P. 190-203.
13. Santos-Montes A., Gonzalo-Lumbreras R., Gasco-Lopez AI. et al. Solvent and solidphase
extraction of natural and synthetic corticoids in human urine // J. Chromatogr. 1994.
V. 652(1). P. 83-89.
14. Shackleton C. Clinical steroid mass spectrometry: a 45-year history culminating in
HPLC-MS/MS becoming an essential tool for patient diagnosis // J. Steroid Biochem Mol
Biol. 2010. V. 121(3-5). P. 481-490.
15. Shackleton C.H.L. Profiling steroid hormones and urinary steroids (review) // J.
Chromatogr. B. 1986. V. 379. P. 91-156.
16. Steroid Analysis / Edited by H.L.J. Makin and D.B. Gower. Springer. 2010. 1224 р.
17.Wu Z., Zhang C., Yang C. et al. Simultaneous quantitative determination of
norgestrel and progesterone in human serum by high-performance liquid chromatography tandem mass spectrometry with atmospheric pressure chemical ionization //Analyst. 2000.
V. 125(12). P. 2201-2205.
18.Zhang S., Mada SR.., Sharma S. et al. Simultaneous quantitation of 17alphahydroxyprogesterone
caproate, 17alpha-hydroxyprogesterone and progesterone in human
plasma using high-performance liquid chromatography-mass spectrometry (HPLCMS/
MS). // J. Pharm. Biomed. Anal. 2008. V. 48(1). P. 1174-1180.
Published
2019-11-19
How to Cite
Dutov, A. A., Nikitin, D. A., Tereshkov, P. P., Kolesnikov, A. D., Sverkunova, A. V., Martynova, A. V., Konovalova, O. N., Fedorova, E. N., Lukyanova, J. L., & Yermolina, A. V. (2019). Extraction technologies for clinical HPLC analysis of serum progesterone, 17α-hydroxyprogesterone and synthetic progestin’s. Sorbtsionnye I Khromatograficheskie Protsessy, 14(2). Retrieved from https://journals.vsu.ru/sorpchrom/article/view/1471
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