Features of negative planar chromatography of polystyrenes
Keywords:
negative planar chromatogpaphy, polystyrene standards, mesoporous silica.
Abstract
The liquid chromatography process of polystyrene standards separation has been studied by negative
planar chromatography (NPC) in comparison with column chromatography at 298 K. The commercial
samples of Silica gel KSK, mesoporous Silica S80 and porous borosilicate Glass were used as sorbents. As
the solvent (eluent) tetrahydrofurane, toluene, mixture of benzene and cyclohexane were served.
The experiment sho ws that the polymer macromolecules are moving faster then the eluent
molecules. Parameter
f R takes a value greater than 1 ( f R > 1) in the case of negative planar chromatography
(NPC). The method of the measurement for the values f R > 1 has been suggested. The comparison of the
calculated retention characteristics k' and p K demonstrated a good accordance of its meanings between
NPC and column chromatography methods of separation of narrow fractions of polystyrenes using
tetrahydrofuran as an eluent and nanoporous silicas – Silica gel KSK, Silochrom S80 and pore-glass MPS35
as a chromatographic packing material
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5. J. Chromatogr. Libr., 1983, V. 22 A, pp.
162-192.
2.Geiss F. Fundamentals of Thin Layer
Chromatography (Planar Chromatography).
Heiderberg, Basel, N.Y.: Huttig Verlag, 1987,
V. 1, 278 p.
3.Halpaap H., Krebs K.-F. Thin-layer
chromatographic and high-performance thinlayer
chromatographic ready-for-use
preparations with concentrating zones, J.
Chromatogr. A, 1977, V.14, pp. 823-853
4.Guiochon G., Siouffi A. Study of
performances of TLC. Spot capacity in TLC. J.
Chromatogr., 1982, V. 245, pp. 1-20.
5.Inagaki H., Tanaka T. Characterization of
copolymers by TLC, GPC and other methods.
Pure and Appl. Chem., 1982, V. 54, pp. 309-
322.
6.Belenky B.G., Gankina E.S., Tennikov
M.B., Vilenchik L.Z. Fundamental aspects of
adsorption chromatography of polymers and
their experimental verification by thin-layer
chromatography. J. Chromatogr. A, 1978, V.
147, pp. 99-110.
7.Ryabinina T.I., Khokhlova T.D., El'tekov
Yu.A. Izuchenie adsorbtsii polimerov metodom
tonkosloinoi i kolonochnoi khromatografii.
Makromolekuly na granitse razdela faz / pod
red. Lipatova Yu.S. / [Study of polymer
adsorption by thin-layer and column
chromatography methods. Molecules at
interface / ed. by Lipatov.Yu.S.] Kiev.:
Naukova dumka, 1971, pp. 133-138.
8.Soczewiсski E., Goіkiewicz W., Szumiіo
H.A simple molecular model of adsorption
chromatography: III. Solvent composition
effects in thin-layer chromatography of acidic
solutes in systems of the type: Electron donor
solvent-polyamide. J. Chromatogr. A, 1969, V.
45, pp. 1-13.
9.Krasikov V.D. Osnovy planarnoi
khromatografii [Fundamentals of planar
chromatography], S-Peterburg.: Khimizdat,
2005, 232 p.
10. Litvinova L.S. Thin-Layer
Chromatography of Poly(Methyl Methacrylate)
Using Binary Mobile Phases with
Methylethylketone as Displacer. J. Planar
Chromatogr., 2012, V. 25, pp. 112-116.
11. Sherma J. Biennial review of planar
chromatography: 2011-2013. Centr. Europ. J.
Chem., 2014, V. 12, pp. 427-452.
12. Eltekova N.A., Eltekov Yu.A. Features
of liquid chromatography at negayive value of
the capacity factor. Rus. J. Phys. Chem. A,
1996, V. 70, pp. 492-496.
13. Eltekova N.A., Eltekov Yu.A. Negative
adsorption and liquid chromatography of
polystyrene on silica. Rus. Chem. Bul., 1996,
V. 45, No. 9, pp. 2090-2093.
14. Eltekova N.A., Eltekov A.Yu. A
comparison of the characteristics of negative
and sieve chromatography of dextranes on
nanoporous silicas. Rus. J. Phys. Chem. A,
2004, V. 78, pp. 1320-1325.
15. Eltekov A. Liquid chromatography of
dextrans on porous silica beds. J. Chromatogr.
A., 2005, V. 1100, pp. 15-19.
16. Berezkin V.G., Kormishkina E.V.
Version of conventional thin-layer
chromatography with a contact-covered
sorption layer. J. Analyt. Chem., 2006, V. 61,
No.10, pp. 991-996.
17. Tuzimski T., Soczewinski E. Use of a
database of plots of pesticide retention (RF)
against mobile-phase composition. P. 1.
Correlation of pesticide retention data in
normal-and reversed-phase systems and their
use to separate a mixture of ten pesticides by
2D TLC. J. Planar Chromatogr., 2003, V. 16,
pp. 263-267.
18. Tuzimski T. Separation of a Mixture of
Eighteen Pesticides by Two-Dimensional Thin Layer Chromatography on a Cyanopropyl-
Bonded Polar Stationary Phase. J. Planar
Chromatogr., 2004, V. 17, pp. 328-334.
19. Sherma J. Basic TLC techniques,
materials, and apparatus. Handbook of thinlayer
chromatography / eds. Sherma J., Fried B.
/ New York: Marcel Dekker Inc., 2003, pp. 16-
61.
20. Jarne C., Cebolla V.L., Membrado L.,
Galvez E.M. et al. Revisiting molecular weight
distribution of polystyrenes using adsorption
high-performance thin-layer chromatography.
J. Planar Chromatogr., 2013, V. 26, pp. 5-13.
21. Wall P.E. Thin-layer chromatography. A
modern practical approach. Cambridge.: Royal
Soc. Chem., 2005, 194 p.
22. Poole C.F., Poole S.K.
Multidimensionality in planar chromatography.
J. Chromatogr., 1995, V. 703, pp. 573-612.
Published
2019-11-20
How to Cite
Eltekov, A. Y., Eltekova, N. A., & Eltekov, Y. A. (2019). Features of negative planar chromatography of polystyrenes. Sorbtsionnye I Khromatograficheskie Protsessy, 14(6). Retrieved from https://journals.vsu.ru/sorpchrom/article/view/1586
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