Regularities of betacyanins chromatographic behavior in reversed-phase HPLC
Keywords:
reversed-phase, HPLC, betacyanins, retention, parameters of chromatographic system, stability.
Abstract
Chromatographic behavior of betanin and isobetanin in reversed-phase HPLC has been
investigated. It has been shown that for optimal betalains hydrophobic retention acidified mobile phases
(рН ~ 2) should be applied. The mobile phase must be modified with addition of 4 ÷ 8 vol.% of CH3CN;
thus stationary phases resistant for phase collapse are to be explored. It must be taken into account during
sample preparation that betacyanins in samples under investigation are more stable at pH 4 ÷ 6.
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References
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применение в химическом анализе // Успехи химии. – 2011. – Т.80, №3. – С. 249-271.
3. Delgado-Vargas F., Jiménez A. R., Paredes-López O. Natural Pigments: Carotenoids,
Anthocyanins, and Betalains — Characteristics, Biosynthesis, Processing, and Stability //
Crit. Rev. Food Sci. Nutr. – 2000. – V.40. – P. 173-289.
4. Дейнека В.И., Хлебников В.А., Перистый В.А., Гостищев И.А., Проворная Т.В.
Особенности хроматографического поведения берберина в условиях обращено-
фазовой ВЭЖХ // Сорбц. и хроматогр. процессы. - 2007. - Т.7, Вып.2. - С. 228-235.
5. Дейнека В.И., Григорьев А.М. Относительный анализ удерживания гликозидов
цианидина // Ж. физ. химии. - 2004. - Т.78, №5. - С.923-926.
6. Swiglo A.G., Szymusiak H., Malinowska P. Betanin, the main pigment of red beet:
Molecular origin of its exceptionally high free radical-scavenging activity // Food
Additives Contaminants. – 2006. - V.23. – P. 1079–1087.
7. Horváth C., Melander W., Molnár I. Solvophobic interactions in liquid
chromatography with nonpolar stationary phases // J. Chromatogr. A. – 1976. – V.125. - P. 129–156.
8. Wybraniec S. Formation of Decarboxylated Betacyanins in Heated Purified
Betacyanin Fractions from Red Beet Root (Beta vulgaris L.) Monitored by LC-MS/MSJ //
J. Agric. Food Chem. – 2005. - V.53. – P. 3483-3487.
9. Przybyciel M., Majors R.E. Phase Collapse in Reversed-Phase LC // LC GC Europe. -
2002. - V.20, N.10. - P. 2-5.
10. Henriette M.C. Betalains: properties, sources, applications, and stability //
International Journal of Food Science and Technology. - 2009. - V. 44. – P. 2365–2376.
11. Wybraniec S. Effect of tetraalkylammonium salts on retention of betacyanins and
decarboxylated betacyanins in ion-pair reversed-phase high-performance liquid
chromatography // J. Chromatogr. A. – 2006. – V.1127. – P. 70–75.
Published
2019-11-21
How to Cite
Saenko, I. I., Deineka, V. I., Deineka, L. A., & Karpeeva, I. E. (2019). Regularities of betacyanins chromatographic behavior in reversed-phase HPLC. Sorbtsionnye I Khromatograficheskie Protsessy, 13(4). Retrieved from https://journals.vsu.ru/sorpchrom/article/view/1671
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