Determination of valproic acid in serum using ion chromatography and capillary electrophoresis
Keywords:
ion chromatography, capillary electrophoresis, valproic acid, serum.
Abstract
Ion chromatographic and capillary electrophoresis methods were developed for the
determination of valproic acid in serum after solvent extraction from serum. It was established that
capillary electophoresis allows to increase the expressity and sensitivity of valproic acid determination in
comparison with ion chromatography. Proposed methods have been applied to analysis of real samples of
patient’s sera from Military-Medical academy and Institute of cerebral (Saint Petersburg).
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References
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Valproic Acid in Serum by Liquid Chromatography-Atmospheric Pressure Chemical
Ionization Mass Spectrometry // Anal. Sci. 2001. V. 17. P. 999-1001.
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K.,Orr J. Capillary gas chromatography-mass spectrometry of valproic acid metabolites in
serum and urine using tert.-butyldimethylsilyl derivatives // J. Chrom. 1986. V. 375. P.
285-298.
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by capillary electrophoresis // J. Chrom. A. 2000. V. 884. P. 261-275.
12. Mayer B.X. How to increase precision in capillary electrophoresis // J. Chrom. A.
2001. V.907. P. 21-37.
13. Комарова Н.В., Каменцев Я.С. Практическое руководство по использованию
систем капиллярного электрофореза «КАПЕЛЬ». Санкт-Петербург. 2006. 210 С.
14. Doble P., Andersson P. Buffered chromate electrolytes for the separation and indirect
absorbance detection of inorganic anions in capillary electrophoresis // Anal. Comm. 1997.
V. 34. P. 351-353.
method for the therapeutic drug monitoring of valproic acid and seven of its metabolites in
human serum application of the assay for a group of pediatric epileptics // J. Chrom. B.
1994. V. 656. P.343-347.
2. Yu D., Gordon J.D., Zheng J., Panesar S.K., Rurak D.W., Riggs K. W., Abbott F. S.
Determination of valproic acid and its metabolites using gas chromatography with massselective
detection: application to serum and urine samples from sheep. // J. Chrom. B.
1995. V. 666. P. 269-275.
3. Darius J. On-column gas chromatographic-mass spectrometric assay for metabolic
profiling of valproate in brain tissue and serum // J. Chrom. B. 1996. V. 682. P. 67-75.
4. Dills R.L., Shen D.D. Methods to reduce background interferences in electron-capture
gas chromatographic analysis of valproic acid and its unsaturated metabolites after
derivatization with pentafluorobenzil bromide // J. Chrom. B. 1997. V. 690. P. 139-152.
5. Nakamura M., Kondo K., Nishioka R, Kawai S. Improved procedure for highperformance
liquid chromatographic determination of valproic acid in serum as its
phenacyl ester // J. Chrom. 1984. V. 310. P. 450-454.
6. Дутов А.А., Летунов В.И., Никитин Д.А., Дуйсебеков М.М. Определение
вальпроевой кислоты в биологических жидкостях методом ВЭЖХ с УФ детекцией и
предколоночной дериватизацией фенацил бромидом // Клиническая
фармакокинетика. 2005. вып.1. С. 34-37.
7. Mino T., Nakajima M., Wakabayashi H., Yamato S., Shimada K. Determination of
Valproic Acid in Serum by Liquid Chromatography-Atmospheric Pressure Chemical
Ionization Mass Spectrometry // Anal. Sci. 2001. V. 17. P. 999-1001.
8. Abbott F.S., Kassam J., Acheampong A., Ferguson S.,Panesar S.,Burton R., Farrell
K.,Orr J. Capillary gas chromatography-mass spectrometry of valproic acid metabolites in
serum and urine using tert.-butyldimethylsilyl derivatives // J. Chrom. 1986. V. 375. P.
285-298.
9. Шпигун О.А., Золотов Ю.А. Ионная хроматография и ее применение в анализе
вод. М. 1990. 199 С.
10. Fritz J.S., Gjerde D.T. Ion chromatography. New York, USA: Wiley-VCH. 2000.
254 P.
11. Fritz J.S. Recent developments in the separation of inorganic and small organic ions
by capillary electrophoresis // J. Chrom. A. 2000. V. 884. P. 261-275.
12. Mayer B.X. How to increase precision in capillary electrophoresis // J. Chrom. A.
2001. V.907. P. 21-37.
13. Комарова Н.В., Каменцев Я.С. Практическое руководство по использованию
систем капиллярного электрофореза «КАПЕЛЬ». Санкт-Петербург. 2006. 210 С.
14. Doble P., Andersson P. Buffered chromate electrolytes for the separation and indirect
absorbance detection of inorganic anions in capillary electrophoresis // Anal. Comm. 1997.
V. 34. P. 351-353.
Published
2019-11-22
How to Cite
Nikitina, T. G., & Adamson, V. G. (2019). Determination of valproic acid in serum using ion chromatography and capillary electrophoresis. Sorbtsionnye I Khromatograficheskie Protsessy, 12(1). Retrieved from https://journals.vsu.ru/sorpchrom/article/view/1752
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