Application of Chromato-Mass-Spectrometry in study of hormones
Keywords:
method GC-MS of gorrmons, metrological characteristics, TMS analogs.
Abstract
By the method of Chromato-Mass-Spectrometry lower limits of detection of natural hormones of
steroid nature and their trimethylsilyl (TMS) derivatives were determined. The lower limit of detection is
markedly reduced when TMS is used. The basic patterns of the primary fragmentation of TMS
derivatives of the studied hormones are shown
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И.И.Дедов // Журн. Рос. хим. об-ва им. Д.И. Менделеева. – 2005. – Т.XLIX,№1. –
С.8-10.
3. Quintana, J.B. Determination of natural and synthetic estrogens in water by gas
chromatography with mass spectrometric detection / J.B. Quintana, J.Carpinteiro, I.
Rodrıguez // Journal of Chromatography A. – 2004. – V. 1024. – P.177–185.
4. Kelly, C. Analysis of steroids in environmental water samples using solid-phase
extraction and ion-trap gas chromatography-mass spectrometry and gas chromatographytandem
mass spectrometry / C. Kelly // Journal of Chromatography A. – 2002. – V. 872. –
P.309–314.
5. Streck, G. Chemical and biological analysis of estrogenic, progestagenic and
androgenic steroids in the environment / G. Streck // Trends in Analytical Chemistry. –
2009. - V. 28, № 6. – P.635-652.
6. Lopez de Alda, M.J. Determination of steroid sex hormones and related synthetic
compounds considered as endocrine disrupters in water by liquid chromatography–diode
array detection–mass spectrometry / M.J. Lopez de Alda, D. Barcelo // Journal of
Chromatography A. – 2000. – V.892. – P. 391–406.
7. Ayotte, C. Testing for natural and synthetic anabolic agents in human urine / C.
Ayotte, D. Goudreault, A. Charlebois // J. Chromatography. – 1996. – V.687. – Р. 3-25.
8. Bowden, J. Enhancement of chemical derivatization of steroids by gas
chromatography/mass spectrometry (GC/MS) / J.A. Bowden, D.M. Colosi, D.C. Mora-
Montero, T.J. Garrett, R.A. Yosta// Journal of Chromatography B. - 2009. - № 877. - P. 3237–3242.
9. Zhang, K. Pitfalls and solution for simultaneous determination of estrone and 17α-
ethinylestradiol by gas chromatography–mass spectrometry after derivatization with N,Obis(
trimethylsilyl)trifluoroacetamide / K.Zhang, Y. Zuo // Analytica Chimica Acta. – 2005. – V.554. – P. 190–196.
10. Zhou, Yi-qi. Formation of multiple trimethylsilyl derivatives in the derivatization of
17α-ethinylestradiol with BSTFA or MSTFA followed by gas chromatography-mass
spectrometry determination / Zhou Yi-qi, Wang Zi-jian, Jia Ning // Journal of
Environmental Sciences. – 2007. – V.19. – P.879–884.
11. Zuo, Y. Microwave-accelerated derivatization for the simultaneous gas
chromatographic–mass spectrometric analysis of natural and synthetic estrogenic steroids /
Y. Zuo, K. Zhang , Y. Lin // Journal of Chromatography A. 2007. – V.1148. – P. 211–218.
12. Соколова, Л.С. Хромато-масс-спектрометрическое определение некоторых
женских половых гормонов стероидного строения и их синтетических аналогов /
Л.С. Соколова, Е.Г. Струкова, О.А. Дукова // Journal of Siberian Federal University.
Chemistry. - 2010. – V.4, № 3. – P.408-412.
13. Лебедев, А.Т. Масс-спектрометрия в органической химии / А.Т. Лебедев. –
М.:БИНОМ. Лаборатория знаний, 2003.-493 с.
Published
2019-11-25
How to Cite
Sokolova, lidya S., & Efremov, A. A. (2019). Application of Chromato-Mass-Spectrometry in study of hormones. Sorbtsionnye I Khromatograficheskie Protsessy, 12(6). Retrieved from https://journals.vsu.ru/sorpchrom/article/view/1894
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