Chromatographic separation and mass spectrometric investigation of peptides great wax moth
Keywords:
Galleria mellonella, MALDI TOF peptides, immunity of insects.
Abstract
We studied the change in peptide composition of haemolymph Galleria mellonella, induced
immunization microorganisms, methods of liquid chromatography and mass spectrometry. Established
that as a result of the immune response there is a lot of new peptide components that were not previously
described for this organism. In the hemolymph of caterpillars were identified as known antimicrobial
peptides. In the antibacterial activity of the fractions studied hemolymph detected unstudied peptides of
interest for studies aimed at studying the immune response and innate immunity in insects.
Downloads
Download data is not yet available.
References
1. Schroder, J. M. Epithelial peptide antibiotics // Biochem.Pharmacol. 1999. №57. Р.
121-134.
2. Bulet, P., Stocklin, R., and Menin, L. Anti-microbial peptides: from invertebrates to
vertebrates // Immunol.Rev. 2004. №198. Р. 169-184.
3. Boman, H. G. Peptide antibiotics and their role in innate immunity //
Annu.Rev.Immunol. 1995. №13. Р. 61-92.
4. Huang, H. J., Ross, C. R., and Blecha, F. Chemoattractant properties of PR-39, a
neutrophil antibacterial peptide // J.Leukoc.Biol. 1997. № 61. Р.624-629.
5. Yang, D., Chertov, O., Bykovskaia, S. N., Chen, Q., Buffo, M. J., Shogan, J.,
Anderson, M., Schroder, J. M., Wang, J. M., Howard, O. M., and Oppenheim, J. J. Betadefensins:
linking innate and adaptive immunity through dendritic and T cell CCR6 //
Science. 1999. №286. Р. 525-528.
6. Yang, D., Chertov, O., and Oppenheim, J. J. (2001) The role of mammalian
antimicrobial peptides and proteins in awakening of innate host defenses and adaptive
immunity // Cell Mol.Life Sci. 2001. №58. Р. 978-989.
7. Tauger, S., Webb, D., Siuzdak, G. Peptide and protein analysis with mass
spectrometry // Spectroscopy. 2002. № 16. Р. 15-28.
8. Schoneich, C., Kwok, S.K., Wilson, G.S. Separation and analysis of peptides and
proteins // Anal. Chem. 1993. № 65. P.67 - 84.
9. Geng, M., Ji, J., Regnier, F. E. Signature – peptide approach to detecting proteins in
complex mixtures. // J. of Chromatogr. 2000. №. 870. P. 295-313.
10. Трещанина, Н.А. Лабораторный практикум по микробиологии / Н.А.
Трещанина, – Самара: Изд-во СамГУ, 1997. Ч.3. С.38
11. Фролов, Ю.П. Математические методы в биологии. ЭВМ и программирование:
теоретические основы и практикум / Ю.П. Фролов. Самара: Изд-во СамГУ, 1997. С.265.
12. Brown SE, Howard A, Kasprzak AB, Gordon KH, East P. The discovery and
analysis of a diverged family of novel antifungal moricin-like peptides in the wax moth
Galleria mellonella. Insect Biochemistry and Molecular Biology Rev 2008. №38. Р. 201–212.
13. Mak, Р. Antibacterial peptides of the moth Galleria mellonella / Р. Mak, D. Chmiel,
G. J. Gacek // J. Acta Biochimica Polonica. 2001. №4. Р. 1191-1195.
14. Lamberty, M. Isolation from the lepidopteran heliohtis vireskins of a novel insekt
defensin with potent antifungal activity / M. Lamberty, S.Ales, S. Uttenweiler-Joseph [et
al] / J. of biological chemistry. 1998. V. 274. №14. Р. 9320-9326.
121-134.
2. Bulet, P., Stocklin, R., and Menin, L. Anti-microbial peptides: from invertebrates to
vertebrates // Immunol.Rev. 2004. №198. Р. 169-184.
3. Boman, H. G. Peptide antibiotics and their role in innate immunity //
Annu.Rev.Immunol. 1995. №13. Р. 61-92.
4. Huang, H. J., Ross, C. R., and Blecha, F. Chemoattractant properties of PR-39, a
neutrophil antibacterial peptide // J.Leukoc.Biol. 1997. № 61. Р.624-629.
5. Yang, D., Chertov, O., Bykovskaia, S. N., Chen, Q., Buffo, M. J., Shogan, J.,
Anderson, M., Schroder, J. M., Wang, J. M., Howard, O. M., and Oppenheim, J. J. Betadefensins:
linking innate and adaptive immunity through dendritic and T cell CCR6 //
Science. 1999. №286. Р. 525-528.
6. Yang, D., Chertov, O., and Oppenheim, J. J. (2001) The role of mammalian
antimicrobial peptides and proteins in awakening of innate host defenses and adaptive
immunity // Cell Mol.Life Sci. 2001. №58. Р. 978-989.
7. Tauger, S., Webb, D., Siuzdak, G. Peptide and protein analysis with mass
spectrometry // Spectroscopy. 2002. № 16. Р. 15-28.
8. Schoneich, C., Kwok, S.K., Wilson, G.S. Separation and analysis of peptides and
proteins // Anal. Chem. 1993. № 65. P.67 - 84.
9. Geng, M., Ji, J., Regnier, F. E. Signature – peptide approach to detecting proteins in
complex mixtures. // J. of Chromatogr. 2000. №. 870. P. 295-313.
10. Трещанина, Н.А. Лабораторный практикум по микробиологии / Н.А.
Трещанина, – Самара: Изд-во СамГУ, 1997. Ч.3. С.38
11. Фролов, Ю.П. Математические методы в биологии. ЭВМ и программирование:
теоретические основы и практикум / Ю.П. Фролов. Самара: Изд-во СамГУ, 1997. С.265.
12. Brown SE, Howard A, Kasprzak AB, Gordon KH, East P. The discovery and
analysis of a diverged family of novel antifungal moricin-like peptides in the wax moth
Galleria mellonella. Insect Biochemistry and Molecular Biology Rev 2008. №38. Р. 201–212.
13. Mak, Р. Antibacterial peptides of the moth Galleria mellonella / Р. Mak, D. Chmiel,
G. J. Gacek // J. Acta Biochimica Polonica. 2001. №4. Р. 1191-1195.
14. Lamberty, M. Isolation from the lepidopteran heliohtis vireskins of a novel insekt
defensin with potent antifungal activity / M. Lamberty, S.Ales, S. Uttenweiler-Joseph [et
al] / J. of biological chemistry. 1998. V. 274. №14. Р. 9320-9326.
Published
2019-11-25
How to Cite
Purygin, P. P., Sribnaya, O. S., Klenow, N. A., Litvinova, E. G., Paramonov, S. A., Buryak, A. K., & Serebryakova, M. V. (2019). Chromatographic separation and mass spectrometric investigation of peptides great wax moth. Sorbtsionnye I Khromatograficheskie Protsessy, 11(1). Retrieved from https://journals.vsu.ru/sorpchrom/article/view/1897
Section
Статьи
