Adsorption of eremomycin on the walls of fused silica and modified capillaries in the process of electrophoretic separation of aromatic acid enantiomers
Keywords:
coated capillaries, eremomycin, adsorption.
Abstract
Methods for the preparation of fused-silica capillaries coated with chitosan, 3-
aminopropyltrimethoxysilane, 3-glicidoxypropyltriethoxysilane and eremomycin were offered. It was
found that the most stable coating consisted of eremomycin covalently bonded to the silica surface
through 3-glicidoxypropyltriethoxysilane. The adsorption of eremomycin on the capillary surface was
studied. It was shown that capillary coating reduced the eremomycin adsorption. To reduce this
phenomenon, the 3-aminopropyltrimethoxysilane-coated capillary was shown to be more effective than
that with eremomycin immobilized through 3-glicidoxypropyltriethoxysilane. Four profens were
enantioseparated in coated capillaries using as less as 1 mM eremomycin
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Press. 462 p.
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Electrophoresis. 2001. V. 22. P. 644-655.
5.Ворошилова О.И., Киселев А.В., Никитин Ю.С. Синтез и исследование
кремнеземных носителей с поверхностью, модифицированной γ –
аминопропильными группами// Коллоидн. журн. 1980. Т. 42. С.223-229.
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with macrocyclic glycopeptide antibiotic eremomycin chemically bonded to silica //
J. Chromatogr. A. 2006. V.1108. P.263-267.
7.Fang N., Zhank H., Li J., Li H.-W. Mobility-based wall adsorption isoterms for
comparing capillary electroforesis with single-molecule observations. // Anal. Chem. A.
2007. V.79. P.6047-6054.
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fused-silica capillaries // J. Chromatogr. A. 1999. V.830. P.415-422.
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capillary electrophoresis using coupled chitosan as coated modifier // Talanta. 2006. V.69.
P.463-468.
10. Буданова Н.Ю., Шаповалова Е.Н., Шпигун О.А. Изучение возможности
использования хитозана в капиллярном электрофорезе // Вестн. Моск. ун-та. Сер. 2.
Химия. 2006. Т.47. C.177-181.
11. Буданова Н.Ю. Капиллярное электрофоретическое разделение энантиомеров
при использовании олиго- и полисахаридных хиральных селекторов. Дисс. … канд.
хим. наук. М.: МГУ. Хим. фак, 2005, 161 с.
Published
2019-11-26
How to Cite
Lebedeva, M. V., Prokhorova, A. F., Shapovalova, E. N., Shigun, O. A., Staroverov, S. M., & Kuznetsov, M. A. (2019). Adsorption of eremomycin on the walls of fused silica and modified capillaries in the process of electrophoretic separation of aromatic acid enantiomers. Sorbtsionnye I Khromatograficheskie Protsessy, 11(5). Retrieved from https://journals.vsu.ru/sorpchrom/article/view/1965
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