Комплексное применение методов диск- электрофореза и гель-фильтрации для анализа влияния оксида углерода на структуру молекул оксигемоглобина человека

Elena A. Kalaeva; Valery G. Artyukhov; Olga V. Putintseva

Elena A. Kalaeva Cand.Biol.Sci., the assistant of the department of biophysics and biotechnology of the biological and soil-science faculty of Voronezh State University, Voronezh, e-mail: kalaevae@gmail.com
Valery G. Artyukhov the Honored worker of a science of the Russian Federation, Dr. Sci. Biol., professor, the head of the department of biophysics and biotechnology, the dean of the biological and soil-science faculty of Voronezh State University, Voronezh
Olga V. Putintseva Dr. Sci. Biol., professor of the department of biophysics and biotechnology of the biological and soil-science faculty of Voronezh State University, Voronezh

Keywords: gel-filtration, hemoglobin, PAAG disc-electrophoresis, molecular weight, olygomerous protein, carbon oxide, fraction composition.

Abstract

Chromatographic and electrophoretic properties of samples of human oxy- and
carboxyhemoglobin and their mixes in the ratio 9:1, 5:5 and 1:9 accordingly has been investigated. Joint
application of methods of PAAG disc-electrophoresis and gel-filtration on sephadex has allowed to
establish, that carbon oxide presence promotes turning apoprotein component of hemoglobin molecules.
Replacement О2 on CO results in decrease of heterogeneity of protein structure: at the
electrophoregrammes of HbCO one fraction is found out only, HbO2 has been represented by four
fractions. In preparations of main fraction of HbO2 and HbCO mixes dimeres peculiar to
carboxyhemoglobin was not revealed, in them prevailed tetramerous and monomerous forms similar like
at HbO2. Thus, the effects caused by presence of HbO2, despite of ninefold prevalence hemoglobin
carboxyform in a mixture of hemoproteins were shown

Downloads

Download data is not yet available.

References

1.Тиунов Л.А. Окись углерода. Промышленные, выхлопные и взрывные газы,
содержащие окись углерода // Руководство по гигиене труда. М., 1963. Т. 2. С. 264-
272.
2.Тиунов Л.А., Кустов В.В. Токсикология окиси углерода. Л., 1969. 288 с.
3.Детерман Г. Гель-хроматография. М., 1970. 252 с.
4.Маурер Г. Диск-электрорфорез. М., 1971. 247 с.
5.Drabkin D.L. The chromatographic and optical properties of haemoglobin of man in
comparison with those of other species // J. Biol. Chem. 1946. V. 164, № 5. P. 703-723.
6.Блюменфельд Л.А. Гемоглобин и обратимое присоединение кислорода. М., 1957.
132 с.
7.Артюхов В.Г., Путинцева О.В., Калаева Е.А. Оценка степени фотоповреждения
хромофоров гемового и глобинового компонентов УФ-облученных молекул и
электрофоретических фракций карбоксигемоглобина человека // Радиац. биология.
Радиоэкология. 2000- Т. 40, № 4. С. 439-445.
8.Вредные вещества в промышленности / под ред. Лазарева Н.В., Гадаскиной И.Д.
Л., 1977. Т. 3. С. 240-253.
9.Лужников Е.А., Костомарова Л.Г. Острые отравления. М., 1989. 432 с.

Complex application of the disc-electrophoresis and gel-filtrations methods for the analysis of carbon oxide influence on the structure of human oxyhemoglobin molecules

Abstract

Downloads

References