Determination of caffeine, theobromine and theophylline in tea by reversed-phase high-performamce liquid chromatography method
Keywords:
HPLC, determination, caffeine, theobromine, theophylline, tea.
Abstract
Chromatographic behavior of methylxanthines – caffeine, theobromine and theophylline – at the
conditions of reversed-phase high-performance liquid chromatography was studied. Conditions of
methylxanthines separation and determination were chosen by the variation of mobile and stationary
phase nature. Developed method was used for caffeine, theobromine and theophylline determination in
the different tea samples
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References
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комплексообразования катехинов с ионами Fe3+ при определении кофеина в чае
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xanthines in teas by an improved high-performance liquid chromatographic method using a
polyvinylpolypyrroridone pre-column // J. Chromatogr. A. 1999. V. 848. P. 523 – 527.
catechins and caffeine in tea. Differentiation of green, black and instant teas // Analyst.
2000. V. 125. P. 421 – 425.
2.Fernandez P.L., Lopez A., Pablos F., Gonzalez A.G., Martin M. J. The use of
catechins and purine alkaloids as descriptors for the differentiation of tea beverages //
Microchim. Acta. 2003. V. 142. P.79– 84.
3.Шафигулин Р.В., Буланова А.В., Ро К.Х. Хроматографический анализ
флавоноидов, содержащихся в чае // Сорбционные и хроматографические процессы.
2006. Т. 6. №5. С. 844 – 850.
4.Шафигулин Р.В., Буланова А.В., Ро К.Х. Качественное и количественное
содержание катехинов в различных сортах чая // Сорбционные и
хроматографические процессы. 2007. Т. 7. №21. С. 349 – 352.
5.Horie H., Kohata K. Analysis of tea by high-performance liguid chromatography and
high-performance capillary electrophoresis // J. Chromatogr. A. 2000. V. 881. P. 425 –
438.
6.Horie H., Nesumi A., Ujihara T., Kohata K. Rapid determination of caffeine in tea
leaves // J. Chromatogr. A. 2001. V.942. P. 271 – 273.
7.Yamauchi Y., Nakamura A., Kohno I., Hatanaka K.. Kitai M., Tanimoto T. Quasi-flow
injection analysis for rapid determination of caffeine in tea using the sample pre-treatment
method with a cartridge column filled with polyvinylpolypyrrolidone // J. Chromatogr. A.
2008. V.1177. P. 190 – 194.
8.Weiss D., Anderton C.R. Determination of catechins in matcha green tea by micellar
electrokinetic chromatography // J. Chromatogr. A. 2003. V.1011. P. 173 – 180.
9.Карцова Л.Ф., Алексеева А.В. Использование селективного
комплексообразования катехинов с ионами Fe3+ при определении кофеина в чае
методом высокоэффективной тонкослойной хроматографии // Журн. аналит. химии.
2009. Т. 64. № 9. С. 954 – 958.
10. Aucamp JP., Hara Y., Apostolides Z. Simultaneous analysis of tea catechins,
caffeine, gallic acid, theanine and ascorbic acid by micellar electrokinetic capillary
chromatography // J. Chromatogr. A. 2000. V.876. P. 235 – 242.
11. Chen Q., Mou S., Hou X., Ni Z. Simultaneous determination of caffeine,
theobromine and theophylline in foods and pharmaceutical by using ion chromatography //
Anal. chim. acta. 1998. V.371. P. 287 – 296.
12. Cardozo Jr. E. L., Ferrarese-Filho O., Filho L. C., Ferrarese M.L.L., Donaduzzi C.M.,
Sturion J.A. Methylxanthines and phenolic compounds in mate (Ilex paraguariensis St.
Hil.) progenies grown in Brazil // J. Food Composition and Analysis. 2007. V. 20. P. 553 –558.
13. Nakakukia H., Horieb H., Yamauchib Yu., Kohata K. Rapid analysis of methylated
xanthines in teas by an improved high-performance liquid chromatographic method using a
polyvinylpolypyrroridone pre-column // J. Chromatogr. A. 1999. V. 848. P. 523 – 527.
Published
2019-11-28
How to Cite
Andreeva, E. Y., Tang, J., Dmitrienko, S. G., & Zolotov, Y. A. (2019). Determination of caffeine, theobromine and theophylline in tea by reversed-phase high-performamce liquid chromatography method. Sorbtsionnye I Khromatograficheskie Protsessy, 10(6). Retrieved from https://journals.vsu.ru/sorpchrom/article/view/2114
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